(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Phaffomycetaceae: NE > Komagataella: NE > Komagataella pastoris: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRYQEVHEPENPFSLLVKGQTIVEIPQVQLESGETLHNVPVAFKTWGKLN KEGTNCMVICHALTGSSDVVDWWGPLLGKGKAFDPSKFFIVCLNSLGSPY GSCSPVTRDPAVNELYGPEFPLVSVRDDVNIHRLILNDLGVKQVAIVIGG SMGGMLALEWCFFGLSYVLNCVVLATSAKHSAWCISWGEAQRQSIYSDPK YDDGYYPLEDPPIQGLGAARMSALLTYRSRNSFEERFGRGAPTPQMREKS LRTSVITDAIEAPAVSEDNCAVHNDGHYSYSSSTKVRSRSLSSNNFSLLD VKEDTSSIVNQHGVGQRTRRRPQHYFSAQSYLRYQAAKFSQRFDANCYIS ITRKLDTHDVGRNRPEFHNSTIEALQSLEQNFLVVGIQSDALFTYSEQVF IAKHVKNSKLCKIDSPEGHDAFLLEFEAINKLILEFEIEHVPELLMQTSD DSWSDSELGNTKESIFGEVEDPTSW
We describe the isolation and characterization of a new biosynthetic gene, MET2, from the methylotrophic yeast Pichia pastoris. The predicted product of PpMET2 is significantly similar to its Saccharomyces cerevisiae counterpart, ScMET2, which encodes homoserine-O-transacetylase. The ScMET2 was able to complement the P. pastoris met2 strain; however, the converse was not true. Expression vectors based on PpMET2 for the intracellular and secreted production of foreign proteins and corresponding auxotrophic strains were constructed and tested for use in heterologous expression. The expression vectors and corresponding strains provide greater flexibility when using P. pastoris for recombinant protein expression.
