(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Phaffomycetaceae: NE > Komagataella: NE > Komagataella pastoris: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Pichia pastoris GS115: N, E.
Komagataella pastoris GS115: N, E.
Komagataella phaffii CBS 7435: N, E.
Komagataella pastoris CBS 7435: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA NSSQDARIADARDIKDIVQFHGYQCKEYVVTTRDGYLLTVHRIYKDKIHD NPVVYLQHGLLTNSELFVLNDSPDKIIPFRLVDNGYDVWLGNNRGNKYSR NHTSISTKSKRFWNFSLNEYAIYNIPDSIRTILHITNKHKILAYIGFSQG TAQGLASLSLNPYLNDKIQLMIGLSPAFIPPNLRHPVASFAVNAYHYLPL VLGNRAIMPSVHFWKWAMGQEYYQRVVDLSLIYLFNWQGDNISSQQKQIG YPHLFSNSSVKSILHWFQIIKNQRFEMFDEKDHDSSPLNVIFGLKGHLPT PFPTNNISTKLLLMYGTNDTLMNIPTMLPYLPHENVLDIVPVQDYEHMDL LWGKNVDKLVIPTILKTLAEQVPLAEQVETVEKRFSKLLAPYMKQPVFEI EGPQRYATIQIS
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production hosts making it well suited for improving the understanding of associated genomic features. Here, we present a 9.35 Mbp high-quality genome sequence of P. pastoris CBS7435 established by a combination of 454 and Illumina sequencing. An automatic annotation of the genome sequence yielded 5007 protein-coding genes, 124 tRNAs and 29 rRNAs. Moreover, we report the complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast. Fifteen genes encoding proteins, 2 rRNA and 25 tRNA loci were identified on the 35.7 kbp circular, mitochondrial DNA. Furthermore, the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.
