(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Phaffomycetaceae: NE > Komagataella: NE > Komagataella pastoris: NE
A85-EsteraseD-FGH : picpa-c1phg5Pichia pastoris (Yeast)(and strain GS115) Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase. abh_upf0017 : picpg-c4r6t8Pichia pastoris (Yeast) Putative acyltransferase with similarity to Eeb1p and Eht1p. Acidic_Lipase : picpg-c4qw07Pichia pastoris (Yeast) Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p), picpg-c4r0k6Pichia pastoris (Yeast) Steryl ester hydrolase, at the plasma membrane, picpg-c4r403Pichia pastoris) (Yeast) Steryl ester hydrolase. Carboxypeptidase_S10 : picpa-cbpyPichia pastoris (Yeast) PRC1 gene, picpa-KEX1Pichia pastoris (Yeast) Komagataella pastoris, carboxypeptidase kex1 precursor. CGI-58_ABHD5_ABHD4 : picpg-c4r1s0Pichia pastoris (strain GS115) (Yeast) Putative uncharacterized protein, picpg-c4r4a5Pichia pastoris (strain GS115) (Yeast) Putative uncharacterized protein, picpg-c4r029Pichia pastoris (strain GS115) (Yeast) Putative uncharacterized protein. DPP4N_Peptidase_S9 : picpg-c4r272Pichia pastoris (Yeast) Dipeptidyl aminopeptidase, Golgi integral membrane protein. Fusarinine_C_esterase_sidJ : picp7-f2qwe3Pichia pastoris (Yeast) UPF0613 protein PB24D3.06c. Homoserine_transacetylase : picpa-q5fy74Pichia pastoris (Yeast) homoserine-o-transacetylase. LIDHydrolase : picpg-c4r7h5Pichia pastoris (strain GS115) (Yeast) Putative uncharacterized protein. Monoglyceridelipase_lysophospholip : picpg-c4r663Pichia pastoris (Yeast) Serine hydrolase with sequence similarity to monoglyceride lipase (MGL). PPase_methylesterase_euk : picpg-c4r788Pichia pastoris) (Yeast) Protein phosphatase methylesterase 1
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Pichia pastoris GS115: N, E.
Komagataella pastoris GS115: N, E.
Komagataella phaffii CBS 7435: N, E.
Komagataella pastoris CBS 7435: N, E.
Komagataella pastoris: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MYPEHKYREYQRRVPLWQYSLLVIVLLYGSHLLISTINLIHYNHKNYHAH PVNSGIVLNEFADDDSFSLNGTLNLENWRNGTFSPKFHSIQWTEIGQEDD QGYYILSSNSSYIVKSLSDPDFESVLFNESTITYNGEEHHVEDVIVSNNL QYALVVTDKRHNWRHSFFANYWLYKVNNPEQVQPLFDTDLSLNGLISLVH WSPDSSQVAFVLENNIYLKHLNNFSDSRIDQLTYDGGENIFYGKPDWVYE EEVFESNSAMWWSPNGKFLSILRTNDTQVPVYPIPYFVQSDAETAIDEYP LLKHIKYPKAGFPNPVVDVIVYDVQRQHISRLPAGDPFYNDENITNEDRL ITEIIWVGDSRFLTKITNRESDLLAFYLVDAEANNSKLVRFQDAKSTKSW FEIEHNTLYIPKDTSVGRAQDGYIDTIDVNGYNHLAYFSPPDNPDPKVIL TRGDWEVVDSPSAFDFKRNLVYFTATKKSSIERHVYCVGIDGKQFNNVTD VSSDGYYSTSFSPGARYVLLSHQGPRVPYQKMIDLVKGTEEIIESNEDLK DSVALFDLPDVKYGEIELEKGVKSNYVEIRPKNFDESKKYPVLFFVYGGP GSQLVTKTFSKSFQHVVSSELDVIVVTVDGRGTGFKGRKYRSIVRDNLGH YESLDQITAGKIWAAKPYVDENRLAIWGWSYGGYMTLKVLEQDKGETFKY GMSVAPVTNWKFYDSIYTERYMHTPQDNPNYYNSSIHEIDNLKGVKRFLL MHGTGDDNVHFQNTLKVLDLFDLHGLENYDIHVFPDSDHSIRYHNGNVIV YDKLFHWIRRAFKAGK
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production hosts making it well suited for improving the understanding of associated genomic features. Here, we present a 9.35 Mbp high-quality genome sequence of P. pastoris CBS7435 established by a combination of 454 and Illumina sequencing. An automatic annotation of the genome sequence yielded 5007 protein-coding genes, 124 tRNAs and 29 rRNAs. Moreover, we report the complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast. Fifteen genes encoding proteins, 2 rRNA and 25 tRNA loci were identified on the 35.7 kbp circular, mitochondrial DNA. Furthermore, the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.
Title: Identification of the dipeptidyl aminopeptidase responsible for N-terminal clipping of recombinant Exendin-4 precursor expressed in Pichia pastoris Prabha L, Govindappa N, Adhikary L, Melarkode R, Sastry K Ref: Protein Expr Purif, 64:155, 2009 : PubMed
Exendin-4 is a naturally occurring 39 amino acid peptide that is useful for the control of Type 2 diabetes. Recombinant Exendin-4, with an extra glycine at the carboxy-terminus (Exdgly), was expressed in the methylotropic yeast Pichia pastoris. A high proportion of the Exdgly molecules secreted into medium were found to be clipped, lacking the first two amino acids (His-Gly) from the N-terminus. Disruption of the P. pastoris homolog of the Saccharomyces cerevisiae dipeptidyl aminopeptidase (STE13) gene in Pichia genome resulted in a clone that expressed N-terminally intact Exdgly. Elimination of N-terminal clipping enhanced the yield and simplified the purification of Exdgly from P. pastoris culture supernatant.
