(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > rosids: NE > fabids: NE > Rosales: NE > Rosaceae: NE > Maloideae: NE > Amygdaleae: NE > Prunus: NE > Prunus armeniaca: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MDVQRLGEMLKISEENLKTSCYLSLKAQKFPGEQFLVEKSPASSHAFFSF PGSWSVDGWYSGDKAFGEKKINLELFPSMKSISNYDKYDKLEDQELATGF VNQAFLHRFEDVLKTSQLVNQVEAALNEQKSIIFTGHSTGGAIAALATIW FLQKYPNANNGAFFCVTFGSPLVGNHIISHALRREKWSQCFIHFVMRYDI VPRILLAPLTSIQEQFEKVLPFFNPASPKFKSEVLGASPEALQFYENVIR NASSLTSHVASQLMGNANLLLDTVKHFTKLSPYKPFGTYVFCTGNGKLVV LKNPEAVLQTLSYSCLLSSETEHAAVIAHKCLNEHFGYEKEFLGDESSLD MQNVVDFDKLELRLGSDGYLDDLGLGVRARLSLCAARKFEEKKQENEKSV ASKVEAHKEEMKKLEDYREFYNGKDGGYYQAFKIQGEKKDFDANVSRLVL AGIWDEIIEMLKKYELPDEFECTDKWIKLGTNFRNLVEPLDIANYYRHAK GRRYMDKGGRPRRYKYAQRWLEHHEKKQPGDCGNSSCHWANEEESLKR
Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing of haploid gametes enabling separation of the whole-genome sequencing reads into haplotype-specific reads sets. After assembling the reads of each haplotype, the contigs are scaffolded to chromosome level using a genetic map derived from the gametes. We assemble the two genomes of a diploid apricot tree based on whole-genome sequencing of 445 individual pollen grains. The two haplotype assemblies (N50: 25.5 and 25.8 Mb) feature a haplotyping precision of greater than 99% and are accurately scaffolded to chromosome-level.
