Gene_locus Report for: psepu-PP4624 Pseudomonas putida (strains KT2440; F1 / ATCC 700007; GB-1) hydrolase, alpha/beta fold family Ppueh
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Gammaproteobacteria: N E > Pseudomonadales: N E > Pseudomonadaceae: N E > Pseudomonas: N E > Pseudomonas putida group: N E > Pseudomonas putida: N E 6_AlphaBeta_hydrolase :
psepg-b0kkc4 Pseudomonas putida (strain GB-1) Putative uncharacterized protein ,
psepu-PHAG Pseudomonas putida, Arthrobacter siderocapsulatus, P. nitroreducens, P. pseudoalcaligenes (stutzeri, mendocina perfectomarina) acyl-transferase (phaG) gene, complete cds ,
psepu-PP0498 Pseudomonas putida (strain KT2440) hypothetical protein ,
psepu-PP1829 Pseudomonas putida (strain KT2440) Pseudomonas putida F1 hydrolase, alpha/beta fold family ,
psepu-PP1979 Pseudomonas putida (strain KT2440) hydrolase, putative ,
psepu-PP4164 Pseudomonas putida (strain KT2440 and strain F1) Pseudomonas putida W619 hydrolase, alpha/beta fold family ,
psepu-PP4540 Pseudomonas putida (strain KT2440 and strain F1) hydrolase, alpha/beta fold family .
A85-EsteraseD-FGH :
psepu-PP1617 Pseudomonas putida (strain KT2440; W619; GB-1; F1 / ATCC 700007) Pseudomonas entomophila (strain L48) esterase, putative .
A85-IroE-IroD-Fes-Yiel :
psepg-b0kl84 Pseudomonas putida (strain GB-1) Putative esterase ,
psepu-PP0532 Pseudomonas putida (strain KT2440; F1 / ATCC 700007) Pseudomonas putida F1 conserved hypothetical protein ,
psepw-b1j3g8 Pseudomonas putida (strain W619) Putative esterase ,
psepw-b1j6i6 Pseudomonas putida (strain W619) Putative esterase .
ABHD13-BEM46 :
psepu-PP1500 Pseudomonas putida, Pseudomonas taiwanensis; Pseudomonas monteilii; Pseudomonas sp. lipoprotein, putative .
abh_upf0017 :
psepu-PP5117 Pseudomonas putida (strains KT2440; F1 / ATCC 700007; GB-1; BIRD-1) Pseudomonas sp. TJI-51, hydrolase, alpha/beta fold family ,
psepw-b1j2f5 Pseudomonas putida (strain W619) Alpha/beta hydrolase fold .
abh_upf00227 :
psepu-PP4916 Pseudomonas putida, Pseudomonas sp.; Pseudomonas taiwanensis; Pseudomonas sp., Pseudomonas monteilii, conserved hypothetical protein .
Aclacinomycin-methylesterase_RdmC :
psepu-PP1064 Pseudomonas putida (strains KT2440; F1 / ATCC 700007; GB-1) hydrolase, alpha/beta fold family ,
psepu-PP2804 Pseudomonas putida (strain KT2440) hydrolase, alpha/beta fold family ,
psepw-b1j4g1 Pseudomonas putida (strain W619) Alpha/beta hydrolase fold .
AcoC_BiotinLipoyl-ABH :
psepu-acoc Pseudomonas putida dihydrolipoamide acetyltransferase (acoC) .
AlphaBeta_hydrolase :
psepu-PP2083 Pseudomonas putida (strain KT2440 and strain K1) lipase, putative ,
psepu-PP2201 Pseudomonas putida (strain KT2440 and strain F1) hydrolase, alpha/beta fold family ,
psepu-PP2567 Pseudomonas putida (strain KT2440 and strain F1) hydrolase, alpha/beta fold family ,
psepu-PP3404 Pseudomonas putida, conserved hypothetical protein ,
psepu-PP4165 Pseudomonas putida (strain KT2440 and strain F1) conserved hypothetical protein ,
psepu-tpes Pseudomonas putida tropinesterase (EC 3.1.1.10) .
Asp2 :
psepu-a0a1n7gzh5 Pseudomonas putida (Arthrobacter siderocapsulatus). Uncharacterised protein family (UPF0227) .
Atu1826-like :
psepu-PP1310 Pseudomonas putida (strain KT2440) Pseudomonas putida F1 conserved hypothetical protein ,
psepu-PP3367 Pseudomonas putida (strain KT2440) Pseudomonas putida F1 conserved hypothetical protein .
Bacterial_lip_FamI.1 :
psepu-LIP Pseudomonas putida (strain KT2440) lipase .
BioH :
psepu-BIOH Pseudomonas putida (strain KT2440) Pseudomonas putida F1 biotin biosynthesis protein bioh ,
psepw-b1jec3 Pseudomonas putida (strain W619; GB-1; F1 / ATCC 700007) Carboxylesterase .
Carbon-carbon_bond_hydrolase :
psepu-bph Pseudomonas putida KF715 2-hydroxy-6-phenylhexa-2,4-dienoic acid hydrolase (bphD) gene ,
psepu-homoda Pseudomonas putida Pseudomonas putida F1 cmtE HOMODA 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase ,
psepu-ipbD Pseudomonas putida 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase (ipbD) ,
psepu-mhpc1 Pseudomonas putida (Arthrobacter siderocapsulatus) cbzf 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 1 ,
psepu-mhpc2 Pseudomonas putida (Arthrobacter siderocapsulatus) 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2 ,
psepu-nahN Pseudomonas putida (Arthrobacter siderocapsulatus), Pseudomonas aeruginosa, hydoroxymuconic semialdehyde hydrolase on plasmid pWW0 ,
psepu-oxoH Pseudomonas putida 2-oxo-1,2-dihydroquinoline 8-monooxygenase oxoH gene ,
psepu-q1xgk7 Pseudomonas putida 2-hydroxymuconic semialdehyde hydrolase nahn ,
psepu-q400k8 Pseudomonas putida hydrolase ,
psepu-TDNF Pseudomonas putida Acidovorax sp. (strain JS42), uncultured bacterium ,2-hydroxymuconic semialdehyde hydrolase ,
psepu-todf Pseudomonas putida Pseudomonas nitroreducens, 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase gene ,
psepu-XYLF Pseudomonas putida 2-hydroxymuconic semialdehyde hydrolase on plasmid pWW0 ,
psepw-b1j6y4 Pseudomonas putida (strain W619) 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase ,
psesp-dmpd Pseudomonas putida 2-hydroxymuconic semialdehyde hydrolase dmpD gene(hmsh) .
Carboxymethylbutenolide_lactonase :
psepw-b1j3t3 Pseudomonas putida (strain W619) 3-oxoadipate enol-lactonase ,
psepu-a0a083utf1 Pseudomonas putida (Arthrobacter siderocapsulatus). 3-oxoadipate enol-lactonase ,
psepu-q9wwz4 Pseudomonas putida (Arthrobacter siderocapsulatus); Pseudomonas monteilii ; Pseudomonas sp.; Pseudomonas taiwanensis; Pseudomonas plecoglossicida, PcaD-like protein .
Dienelactone_hydrolase :
psepu-clcd1 ,
psepu-PP1184 Pseudomonas putida, Pseudomonas sp., dienelactone hydrolase family protein ,
psepu-PP4178 Pseudomonas putida, dienelactone hydrolase family protein ,
psepu-q7m180 Pseudomonas putida hypothetical 23.8k protein .
Duf_726 :
psepg-b0kiv4 Pseudomonas putida (strain GB-1) Putative uncharacterized protein .
Duf_1100-S :
psepu-PP3195 Pseudomonas putida (strain KT2440) conserved hypothetical protein .
Duf_3530 :
psepg-b0kja0 Pseudomonas putida (strain GB-1) Putative uncharacterized protein ,
psepk-q88qt0 Pseudomonas putida Putative uncharacterized protein ,
psepw-b1je47 Pseudomonas putida (strain W619) Putative uncharacterized protein .
Epoxide_hydrolase :
psepg-b0klt5 Pseudomonas putida (strain GB-1) Alpha/beta hydrolase fold ,
psepu-PP3645 Pseudomonas putida (strain KT2440 and strain F1/ ATCC 700007) hydrolase, alpha/beta fold family .
Fungal-Bact_LIP :
psepu-a0a160gpc6 Pseudomonas putida (Arthrobacter siderocapsulatus) lip-PC2 Thermo-Stable Lipase ,
psepg-b0kir7 Pseudomonas putida (strain GB-1) Pseudomonas entomophila (strain L48) Secretory lipase .
Haloperoxidase :
psep1-a5wax1 Pseudomonas putida W619 (strain F1 / ATCC 700007) Alpha/beta hydrolase fold ,
psepu-bpest ,
psepu-bpoest Pseudomonas putida gene for bromoperoxidase-esterase .
HOD-cofactorfree-dioxygenase :
psepu-QDO .
Homoserine_transacetylase :
psepu-METX Pseudomonas putida (strains KT2440; F1 / ATCC 700007; GB-1; W619) Pseudomonas entomophila (strain L48) homoserine o-acetyltransferase ,
psepu-METXb Pseudomonas putida homoserine-ortho-acyltransferase .
Hormone-sensitive_lipase_like :
psepu-PP3812 Pseudomonas putida, Esterase EstK lipase, putative ,
psepu-Q8KQK1 Pseudomonas putida esterase ,
psepu-q76hn7 Pseudomonas putida probable esterase ,
psepu-a0a177ywl5 Pseudomonas putida (Arthrobacter siderocapsulatus). Alpha/beta hydrolase ,
psepu-a0a1b2f5w1 Pseudomonas putida (Arthrobacter siderocapsulatus) Carboxylesterase NlhH ,
psepu-a0a1c1b7w1 Pseudomonas putida (Arthrobacter siderocapsulatus) Lipase ,
psepu-a0a160grq4 Pseudomonas putida (Arthrobacter siderocapsulatus) Lipase .
Hydrolase_RBBP9_YdeN :
psepu-PP5167 Pseudomonas putida, conserved hypothetical protein .
LYsophospholipase_carboxylesterase :
psepu-ESTB Pseudomonas putida, Pseudomonas sp., carboxylesterase .
MEST-like :
psepw-b1j387 Pseudomonas putida (strain W619) Alpha/beta hydrolase fold .
Monoglyceridelipase_lysophospholip :
psepg-b0kp39 Pseudomonas putida (strain GB-1) Lipase ,
psepu-PP4551 Pseudomonas putida (Arthrobacter siderocapsulatus), Pseudomonas entomophila, hydrolase, alpha/beta fold family ,
psepu-PP5161 Pseudomonas putida, Pseudomonas sp., conserved hypothetical protein ,
psepw-b1j296 Pseudomonas putida (strain W619) Lipase .
NFM-deformylase :
psep6-f8g0m2 ,
psepu-PP3943 Pseudomonas putida N-formylmaleamic acid deformylase, alpha/beta fold family ,
psepw-b1j7g1 Pseudomonas putida (strain W619). Alpha/beta hydrolase fold .
NLS3-Tex30 :
psepu-PP4249 Pseudomonas putida conserved hypothetical protein .
PHA_depolymerase_arom :
psepu-PHAZ Pseudomonas putida, Pseudomonas sp., Pseudomonas entomophila, pha depolymerase poly(3-hydroxyalkanoate)depolymerase (phaZ) ,
psepu-q6pli2 Pseudomonas putida polyhydroxyalkanote depolymerase .
PHA_synth_I :
psepu-PHBC Pseudomonas putida Pseudomonas sp. 10c-1-3 poly(3-hydroxyalkanoate) phb synthase .
PHA_synth_II :
psepu-PHAC1 Pseudomonas putida (Arthrobacter siderocapsulatus), Pseudomonas sp., pha synthase 1 poly(3-hydroxyalkanoate) polymerase 1 ,
psepu-PHAC2 Pseudomonas putida (Arthrobacter siderocapsulatus) poly(3-hydroxyalkanoate) synthase 2 .
PhoPQ_related :
psep1-a5wa77 Pseudomonas putida (strains F1 / ATCC 700007; KT2440) PhoPQ-activated pathogenicity-related protein-like protein .
PMH_Peptidase_S9 :
psepu-PP0375 Pseudomonas putida (strain KT2440 and strain F1) prolyl oligopeptidase family protein .
Proline_iminopeptidase :
psepu-PIP Pseudomonas putida proline iminopeptidase .
Prolyl_oligopeptidase_S9 :
psepu-PP2236 Pseudomonas putida (strain KT2440) prolyl oligopeptidase family protein .
RsbQ-like :
psepg-b0kfg1 Pseudomonas putida (strain GB-1) Alpha/beta hydrolase fold .
S9N_PREPL_Peptidase_S9 :
psepu-PP4583 Pseudomonas putida, peptidase, putative .
T6SS-TLE1 :
psepg-b0kg49 Pseudomonas putida (strain GB-1). Rhs element Vgr protein .
Thioesterase :
psepu-PP3807 Pseudomonas putida, thioesterase ,
psepu-PPSD Pseudomonas putida, non-ribosomal siderophore peptide synthetase thioesterase domain .
UCP031982 :
psepu-PP2934 Pseudomonas putida (strains KT2440; F1/ATCC700007; GB-1) hypothetical protein .
UCP037442 :
psepg-b0kgw1 Pseudomonas putida (strain GB-1) Alpha/beta hydrolase fold ,
psepu-PP4634 Pseudomonas putida, conserved hypothetical protein ,
psepw-b1j2v7 Pseudomonas putida (strain W619) Alpha/beta hydrolase fold .
VirJ :
psepk-q88nk6 Pseudomonas putida, Pseudomonas entomophila, Pseudomonas sp., hypothetical protein ,
psepu-a0a059uyg1 Pseudomonas putida (Arthrobacter siderocapsulatus); Pseudomonas plecoglossicida; Pseudomonas sp.; Pseudomonas monteilii; Pseudomonas taiwanensis. Virulence factor ,
psepu-a0a083uhh3 Pseudomonas putida (Arthrobacter siderocapsulatus); Pseudomonas umsongensis; Pseudomonas sp. Virulence factor family protein ,
psepu-a0a0d1ldt1 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepu-a0a0m3cvc6 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepu-a0a126s8q4 Pseudomonas putida (Arthrobacter siderocapsulatus); Pseudomonas sp.; Pseudomonas mosselii. Virulence factor family protein ,
psepu-a0a160gru2 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepu-a0a177sr79 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepu-a0a177zbe8 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepu-a0a1b2f9z9 Pseudomonas putida (Arthrobacter siderocapsulatus). Bacterial virulence protein ,
psepu-a0a1j1ar09 Pseudomonas putida (Arthrobacter siderocapsulatus). Virulence factor family protein ,
psepw-b1jd86 Pseudomonas putida (strain W619). Virulence factor family protein Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can
retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Pseudomonas putida KT2440:
N ,
E .
Pseudomonas putida GB-1:
N ,
E .
Pseudomonas putida F1:
N ,
E .
Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: psepu-PP4624 Colored MSA for MEST-like (raw)
MAEIPLCVWRTRAQSFMFRGQSIRYWTAGQGEPLLLLHGFPTASWDWHYL
WGPLSQRFRVIACDMLGFGDSDKPVDHTYSLMEQADLQQALLVHLQVAQP
VHLLAHDYGGSVAQELLARHHEQRADIASCVFLNSGLFPESCRMLLIQKL
LLSRFGWLVGRSFGRDDLVRNVMQVYGPCTHPSESALDDFWSLIAANRGT
RILHKLVGYMPERRLHRERWVGAMQRKGVPLRLINGVVDPLSGAHMLERY
RQLVPEPDTVQLLGIGHYPHTEAPVQVLRHYLAFREQPLSFYPPKVAWS
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M A E I P L C V W R T R A Q S F M F R G Q S I R Y W T A G Q G E P L L L L H G F P T A S W D W H Y L W G P L S Q R F R V I A C D M L G F G D S D K P V D H T Y S L M E Q A D L Q Q A L L V H L Q V A Q P V H L L A H D Y G G S V A Q E L L A R H H E Q R A D I A S C V F L N S G L F P E S C R M L L I Q K L L L S R F G W L V G R S F G R D D L V R N V M Q V Y G P C T H P S E S A L D D F W S L I A A N R G T R I L H K L V G Y M P E R R L H R E R W V G A M Q R K G V P L R L I N G V V D P L S G A H M L E R Y R Q L V P E P D T V Q L L G I G H Y P H T E A P V Q V L R H Y L A F R E Q P L S F Y P P K V A W S
References
Title: Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysisvan Loo B , Kingma J , Arand M , Wubbolts MG , Janssen DB Ref: Applied Environmental Microbiology, 72 :2905, 2006 : PubMed Abstract ESTHER: van Loo_2006_Appl.Environ.Microbiol_72_2905 PubMedSearch: van Loo 2006 Appl.Environ.Microbiol 72 2905 PubMedID: 16597997 Gene_locus related to this paper: agrra-echA ,
bacsu-yfhM ,
corsp-cEH ,
deira-DR2549 ,
myctu-ephF ,
nosp7-b2j4w6 ,
nosp7-b2iv02 ,
psepu-PP4624 ,
rhopa-q6n645 ,
strco-2SCG4.13 ,
thefy-q47qj2 Abstract
Epoxide hydrolases play an important role in the biodegradation of organic compounds and are potentially useful in enantioselective biocatalysis. An analysis of various genomic databases revealed that about 20% of sequenced organisms contain one or more putative epoxide hydrolase genes. They were found in all domains of life, and many fungi and actinobacteria contain several putative epoxide hydrolase-encoding genes. Multiple sequence alignments of epoxide hydrolases with other known and putative alpha/beta-hydrolase fold enzymes that possess a nucleophilic aspartate revealed that these enzymes can be classified into eight phylogenetic groups that all contain putative epoxide hydrolases. To determine their catalytic activities, 10 putative bacterial epoxide hydrolase genes and 2 known bacterial epoxide hydrolase genes were cloned and overexpressed in Escherichia coli. The production of active enzyme was strongly improved by fusion to the maltose binding protein (MalE), which prevented inclusion body formation and facilitated protein purification. Eight of the 12 fusion proteins were active toward one or more of the 21 epoxides that were tested, and they converted both terminal and nonterminal epoxides. Four of the new epoxide hydrolases showed an uncommon enantiopreference for meso-epoxides and/or terminal aromatic epoxides, which made them suitable for the production of enantiopure (S,S)-diols and (R)-epoxides. The results show that the expression of epoxide hydrolase genes that are detected by analyses of genomic databases is a useful strategy for obtaining new biocatalysts.
Title: Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440Nelson KE , Weinel C , Paulsen IT , Dodson RJ , Hilbert H , Martins dos Santos VA , Fouts DE , Gill SR , Pop M and Fraser CM <33 more author(s)> Nelson KE , Weinel C , Paulsen IT , Dodson RJ , Hilbert H , Martins dos Santos VA , Fouts DE , Gill SR , Pop M , Holmes M , Brinkac L , Beanan M , DeBoy RT , Daugherty S , Kolonay J , Madupu R , Nelson W , White O , Peterson J , Khouri H , Hance I , Chris Lee P , Holtzapple E , Scanlan D , Tran K , Moazzez A , Utterback T , Rizzo M , Lee K , Kosack D , Moestl D , Wedler H , Lauber J , Stjepandic D , Hoheisel J , Straetz M , Heim S , Kiewitz C , Eisen JA , Timmis KN , Dusterhoft A , Tummler B , Fraser CM (- 33) Ref: Environ Microbiol, 4 :799, 2002 : PubMed Abstract ESTHER: Nelson_2002_Environ.Microbiol_4_799 PubMedSearch: Nelson 2002 Environ.Microbiol 4 799 PubMedID: 12534463 Gene_locus related to this paper: psep1-a5wa77 ,
psep1-a5wax1 ,
psepk-q88nk6 ,
psepk-q88qt0 ,
psepu-acoc ,
psepu-BIOH ,
psepu-bpest ,
psepu-ESTB ,
psepu-LIP ,
psepu-METX ,
psepu-PHAC1 ,
psepu-PHAC2 ,
psepu-PHAG ,
psepu-PHAZ ,
psepu-PIP ,
psepu-PP0375 ,
psepu-PP0498 ,
psepu-PP0532 ,
psepu-PP1064 ,
psepu-PP1184 ,
psepu-PP1310 ,
psepu-PP1500 ,
psepu-PP1617 ,
psepu-PP1829 ,
psepu-PP1979 ,
psepu-PP2083 ,
psepu-PP2201 ,
psepu-PP2236 ,
psepu-PP2567 ,
psepu-PP2804 ,
psepu-PP2934 ,
psepu-PP3195 ,
psepu-PP3367 ,
psepu-PP3404 ,
psepu-PP3645 ,
psepu-PP3807 ,
psepu-PP3812 ,
psepu-PP3943 ,
psepu-PP4164 ,
psepu-PP4165 ,
psepu-PP4178 ,
psepu-PP4249 ,
psepu-PP4540 ,
psepu-PP4551 ,
psepu-PP4583 ,
psepu-PP4624 ,
psepu-PP4634 ,
psepu-PP4916 ,
psepu-PP5117 ,
psepu-PP5161 ,
psepu-PP5167 ,
psepu-PPSD ,
psepu-Q8KQK1 ,
psepu-q9wwz4 Abstract
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.
Other Papers
Pseudomonas putida Pseudomonas sp. B13 dienelactone hydrolase clcD gene