Gene_locus Report for: pyrtt-e3s3p6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Putative uncharacterized protein
Comment
only n-term Pfam A DUF2235 42 422 Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Ascomycota: N E > saccharomyceta: N E > Pezizomycotina: N E > leotiomyceta: N E > dothideomyceta: N E > Dothideomycetes: N E > Pleosporomycetidae: N E > Pleosporales: N E > Pleosporineae: N E > Pleosporaceae: N E > Pyrenophora: N E > Pyrenophora teres: N E > Pyrenophora teres f. teres: N E
ABHD11-Acetyl_transferase :
pyrtt-e3rpq8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Putative uncharacterized protein .
Acetylxylan_esterase :
pyrtt-e3rwh1 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis Putative uncharacterized protein ,
pyrtt-e3rtw2 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s3k4 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s9z0 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein .
Acidic_Lipase :
pyrtt-e3s5q8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s292 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) Lysosomal acid lipase/cholesteryl ester hydrolase .
Arb2_domain :
pyrtt-e3rx11 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Uncharacterized protein .
BD-FAE :
pyrtt-e3rs40 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein .
Canar_LipB :
pyrtt-e3rxj8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)(Drechslera teres f. teres) Putative uncharacterized protein .
Cutinase :
pyrtt-e3ru14 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein ,
pyrtt-e3rg15 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein ,
pyrtt-e3rxy1 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rnt0 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rs45 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Pyrenophora tritici-repentis Putative uncharacterized protein .
Duf_1749 :
pyrtt-e3rkr9 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein .
FaeC :
pyrtt-e3s4g0 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Uncharacterized protein .
Fungal_carboxylesterase_lipase :
pyrtt-e3rcw4 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rfb1 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rh98 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3ril1 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rjq5 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rkg6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rl87 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rp29 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rpe8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rqg8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rrt3 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rth6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rtt7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3ruq0 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3ruw6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rwp7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s2m6 Pyrenophora teres f. teres (Barley net blotch fungus) (Drechslera teres f. teres); Pyrenophora tritici-repentis (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein ,
pyrtt-e3s8r6 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s479 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s701 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s716 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein .
Kynurenine-formamidase :
pyrtt-e3rqx0 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). N-formylkynurenine formamidase .
MpaH :
pyrtt-e3rqw7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). AB hydrolase-1 domain-containing protein .
Prolylcarboxypeptidase :
pyrtt-e3rfu0 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rvm2 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3rxf7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3s922 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein .
Steryl_acetyl_hydrolase :
pyrtt-e3rgg7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)(Drechslera teres f. teres) Putative uncharacterized protein ,
pyrtt-e3sa06 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein .
Thiohydrolase :
pyrtt-e3rgu8 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres). Hydrolase_4 domain-containing protein
Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: pyrtt-e3s3p6 Colored MSA for Duf_2235 (raw)
MASKDTEQKNSSNISPRVFVSTPNNNPMDYGAVSRQQGHVTKKFVLCFDG
TGNKFSGTDADSNILKIYRMLDRNGDDQFHYYQPGIGTYVTKADGSMTRT
GRWEKFQSWYAKAKDSAIGTSFDMHVMAGYRFLMRYYTPGDDIYFFGFSR
GAYTARFLAEMLDHVGLLSAGNEEMCQFAWKTFQKWQCRLPDEKRKDRTP
GEKSNKHKLLEFMCAFRETFSRPVKPIRFLGLFDTVNSVPAFENAWMQRS
RFPYTARSSAVVIRHAVAIDERRAKFRQDLVSQEKPDRSMYYKRRHKHLQ
HMKEMAHDCTERQFMDEKDKEEQNNRGRPETLAPPELFRNPHDTSGVRSQ
SRGYSSDGNFRSGTPSMRSYEGTHTFNSEEEVQDIQEVWFPGCHADIGGG
WPLGDDDAALSHVPLVWIVREA
Legend
This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
M A S K D T E Q K N S S N I S P R V F V S T P N N N P M D Y G A V S R Q Q G H V T K K F V L C F D G T G N K F S G T D A D S N I L K I Y R M L D R N G D D Q F H Y Y Q P G I G T Y V T K A D G S M T R T G R W E K F Q S W Y A K A K D S A I G T S F D M H V M A G Y R F L M R Y Y T P G D D I Y F F G F S R G A Y T A R F L A E M L D H V G L L S A G N E E M C Q F A W K T F Q K W Q C R L P D E K R K D R T P G E K S N K H K L L E F M C A F R E T F S R P V K P I R F L G L F D T V N S V P A F E N A W M Q R S R F P Y T A R S S A V V I R H A V A I D E R R A K F R Q D L V S Q E K P D R S M Y Y K R R H K H L Q H M K E M A H D C T E R Q F M D E K D K E E Q N N R G R P E T L A P P E L F R N P H D T S G V R S Q S R G Y S S D G N F R S G T P S M R S Y E G T H T F N S E E E V Q D I Q E V W F P G C H A D I G G G W P L G D D D A A L S H V P L V W I V R E A
Reference
Title: A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres
Ellwood SR , Liu Z , Syme RA , Lai Z , Hane JK , Keiper F , Moffat CS , Oliver RP , Friesen TL
Ref: Genome Biol, 11 :R109, 2010 : PubMed Abstract ESTHER: Ellwood_2010_Genome.Biol_11_R109 PubMedSearch: Ellwood 2010 Genome.Biol 11 R109 PubMedID: 21067574 Gene_locus related to this paper: pyrtr-b2vv71 ,
pyrtr-b2w1y6 ,
pyrtr-b2w948 ,
pyrtr-b2wbz8 ,
pyrtr-b2wcq8 ,
pyrtr-b2wgg5 ,
pyrtr-dapb ,
pyrtt-e3rcw4 ,
pyrtt-e3rfb1 ,
pyrtt-e3rfu0 ,
pyrtt-e3rgg7 ,
pyrtt-e3rh98 ,
pyrtt-e3ril1 ,
pyrtt-e3rjq5 ,
pyrtt-e3rkg6 ,
pyrtt-e3rkr9 ,
pyrtt-e3rl87 ,
pyrtt-e3rp29 ,
pyrtt-e3rpe8 ,
pyrtt-e3rqg8 ,
pyrtt-e3rrt3 ,
pyrtt-e3rs40 ,
pyrtt-e3rth6 ,
pyrtt-e3rtt7 ,
pyrtt-e3ruq0 ,
pyrtt-e3ruw6 ,
pyrtt-e3rvm2 ,
pyrtt-e3rwp7 ,
pyrtt-e3rxf7 ,
pyrtt-e3s2m6 ,
pyrtt-e3s5q8 ,
pyrtt-e3s8r6 ,
pyrtt-e3s292 ,
pyrtt-e3s479 ,
pyrtt-e3s701 ,
pyrtt-e3s716 ,
pyrtt-e3s922 ,
pyrtt-e3sa06 ,
pyrtt-e3ru14 ,
pyrtt-e3rg15 ,
pyrtt-e3rxy1 ,
pyrtr-b2vyg1 ,
pyrtt-e3rnt0 ,
pyrtt-e3rwh1 ,
pyrtt-e3rtw2 ,
pyrtt-e3s3k4 ,
pyrtt-e3rs45 ,
pyrtr-b2w571 ,
pyrtt-e3s9z0 ,
pyrtt-e3rxj8 ,
pyrtt-e3s3p6 ,
pyrtt-e3rqx0 ,
pyrtt-e3rpq8 ,
pyrtt-e3rx11 ,
pyrtr-b2vzr5 ,
pyrtt-e3s4g0 ,
pyrtt-e3rqw7 ,
pyrtt-e3rgu8 Abstract
BACKGROUND: Pyrenophora teres f. teres is a necrotrophic fungal pathogen and the cause of one of barley's most important diseases, net form of net blotch. Here we report the first genome assembly for this species based solely on short Solexa sequencing reads of isolate 0-1. The assembly was validated by comparison to BAC sequences, ESTs, orthologous genes and by PCR, and complemented by cytogenetic karyotyping and the first genome-wide genetic map for P. teres f. teres. RESULTS: The total assembly was 41.95 Mbp and contains 11,799 gene models of 50 amino acids or more. Comparison against two sequenced BACs showed that complex regions with a high GC content assembled effectively. Electrophoretic karyotyping showed distinct chromosomal polymorphisms between isolates 0-1 and 15A, and cytological karyotyping confirmed the presence of at least nine chromosomes. The genetic map spans 2477.7 cM and is composed of 243 markers in 25 linkage groups, and incorporates simple sequence repeat markers developed from the assembly. Among predicted genes, non-ribosomal peptide synthetases and efflux pumps in particular appear to have undergone a P. teres f. teres-specific expansion of non-orthologous gene families. CONCLUSIONS: This study demonstrates that paired-end Solexa sequencing can successfully capture coding regions of a filamentous fungal genome. The assembly contains a plethora of predicted genes that have been implicated in a necrotrophic lifestyle and pathogenicity and presents a significant resource for examining the bases for P. teres f. teres pathogenicity.
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