(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Mammalia: NE > Theria: NE > Eutheria: NE > Boreoeutheria: NE > Euarchontoglires: NE > Glires: NE > Lagomorpha: NE > Leporidae: NE > Oryctolagus: NE > Oryctolagus cuniculus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAHMLLLWALPLLLGAVAGLEVCYERLGCFGNESPWGGTLERPFSTLPST PKIVNTRFLLYTNENPNNFQEISADASTIRGSNFRTDRKTRFIIHGFTDK GEENWLSNLCENLFQVETVNCICVDWKGGSRTTYPQATQNIRIVGAEVAY LVGTLQSSLGYSPSNIHVIGHSLGAHAAGEVGRRTNGAIGRITGLDPAEP YFQGTPEIVRLDPSDAQFVDVIHTDAAPMVPNLGFGMSQTVGHLDFFPNG GKEMPGCQKNVLSQIVDINGIWEGTRDFVACNHLRSYKYYADSIVNPNGF AGFSCASYTAFTQNKCFPCSNGDCPQMGHYADRFSRKTDGVGQTFYLNTG DSSNFARWRYQVAVTLSGRRVTGHVLVSLYGSKGNSKQYEIFTGLLKPGD THLNEFDSDVDVGDVQKVKFVWYNNVINPTLPKVGASQITVEQNDGRVFK FCSTDTVREDILLTLTPC
The comparison of related genomes has emerged as a powerful lens for genome interpretation. Here we report the sequencing and comparative analysis of 29 eutherian genomes. We confirm that at least 5.5% of the human genome has undergone purifying selection, and locate constrained elements covering approximately 4.2% of the genome. We use evolutionary signatures and comparisons with experimental data sets to suggest candidate functions for approximately 60% of constrained bases. These elements reveal a small number of new coding exons, candidate stop codon readthrough events and over 10,000 regions of overlapping synonymous constraint within protein-coding exons. We find 220 candidate RNA structural families, and nearly a million elements overlapping potential promoter, enhancer and insulator regions. We report specific amino acid residues that have undergone positive selection, 280,000 non-coding elements exapted from mobile elements and more than 1,000 primate- and human-accelerated elements. Overlap with disease-associated variants indicates that our findings will be relevant for studies of human biology, health and disease.
Title: Molecular cloning and characterization of rabbit pancreatic triglyceride lipase Aleman-Gomez JA, Colwell NS, Sasser T, Kumar VB Ref: Biochemical & Biophysical Research Communications, 188:964, 1992 : PubMed
Pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) has been cloned from a gt11 cDNA library made from poly A+ RNA of adult rabbit pancreas. Pancreatic lipase (PL) assists the absorption of dietary triglycerides by hydrolyzing them at 1 and 3 positions to free fatty acids and 2-monoacylglycerol in the presence of bile acids and colipase in the intestinal lumen. Since rabbits are classifically used for the study of the diet induced changes in the lipid metabolism, as a prelude to studying the diet and age dependent changes in the expression of this enzyme, a full length PL cDNA clone was obtained from its pancreas. The coding region of rabbit pancreatic lipase cDNA consists of 1407 base pairs contained in an open reading frame encoding 469 amino acids including the 16 that constitute the signal peptide. Northern blot analysis revealed a band around 1.5 kb. When rabbit enzyme is compared to other species, an over all homology of 70-80% was observed at the nucleotide level. High homology in the amino acid sequence and composition is also apparent between rabbit and other species like dog (65%), pig (76%) and rat (63%). Highest homology is found to be around active-site serine. The regions of homology with other species may help to define sites of interaction of lipase with co-lipase.
Oryctolagus cuniculus protein for liver 60 kD carboxylesterase 1