(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Proteobacteria: NE > Alphaproteobacteria: NE > Rhizobiales: NE > Rhizobiaceae: NE > Rhizobium/Agrobacterium group: NE > Rhizobium: NE > Rhizobium leguminosarum: NE > Rhizobium leguminosarum bv. viciae: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Rhizobium leguminosarum bv. viciae 3841: N, E.
Rhizobium leguminosarum bv. trifolii WSM1325: N, E.
Rhizobium leguminosarum bv. trifolii WSM2304: N, E.
Rhizobium leguminosarum: N, E.
Rhizobium leguminosarum bv. trifolii: N, E.
Rhizobium leguminosarum bv. viciae WSM1455: N, E.
Rhizobium leguminosarum bv. trifolii WSM2297: N, E.
Rhizobium leguminosarum bv. trifolii WSM2012: N, E.
Rhizobium leguminosarum bv. trifolii WSM1689: N, E.
Rhizobium leguminosarum bv. phaseoli CCGM1: N, E.
Rhizobium leguminosarum bv. trifolii WU95: N, E.
Rhizobium leguminosarum bv. trifolii WSM597: N, E.
Rhizobium leguminosarum bv. trifolii CB782: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MIRTILLATACACMLAAAPAGAAEETTQSFETGLIPSPHIFLPEGEVKGT VMLISDAAGWGDYEKGEADRLVAEGAVVIGVDFPSYIQALNQYDVSLNDG CVYMVSDIESLSQQVQRATGNNAYHLPIVAGIGEGGALALAIAAQTPDAT IGQTLAVNPVAGIPLAKELCTPASKQVVGERTVYGLSDGVLPDPIITVFT PDANKDGRAHAEALKKAHDEIEIRDSTDDAQTAFADTLDDLVTASGAFGN PLGLPLAVLEATPAFDTMAVIYSGDGGWRDIDKEVGGTLQKEGIPVVGVD SLHYFWSERKPEETAADLSKIIDFYRKQWKVKHVLLVGYSFGADVVPATY QLLKPAEKSAVAQMSLLSLSHQVDYVISVLGWLGQKTEGAGGDPVGDLKN IDPKLVQCIYGKDDDDDVACPALKDSGAEVIELPGDHHFDENYDLLTKTI IDGLKKRLQN
Rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual Trifolium (clover) species. Strain WSM1325 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from root nodules collected in 1993 from the Greek Island of Serifos. WSM1325 is produced commercially in Australia as an inoculant for a broad range of annual clovers of Mediterranean origin due to its superior attributes of saprophytic competence, nitrogen fixation and acid-tolerance. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a microsymbiont of annual clovers. We reveal that its genome size is 7,418,122 bp encoding 7,232 protein-coding genes and 61 RNA-only encoding genes. This multipartite genome contains 6 distinct replicons; a chromosome of size 4,767,043 bp and 5 plasmids of size 828,924 bp, 660,973 bp, 516,088 bp, 350,312 bp and 294,782 bp.
BACKGROUND: Rhizobium leguminosarum is an alpha-proteobacterial N2-fixing symbiont of legumes that has been the subject of more than a thousand publications. Genes for the symbiotic interaction with plants are well studied, but the adaptations that allow survival and growth in the soil environment are poorly understood. We have sequenced the genome of R. leguminosarum biovar viciae strain 3841. RESULTS: The 7.75 Mb genome comprises a circular chromosome and six circular plasmids, with 61% G+C overall. All three rRNA operons and 52 tRNA genes are on the chromosome; essential protein-encoding genes are largely chromosomal, but most functional classes occur on plasmids as well. Of the 7,263 protein-encoding genes, 2,056 had orthologs in each of three related genomes (Agrobacterium tumefaciens, Sinorhizobium meliloti, and Mesorhizobium loti), and these genes were over-represented in the chromosome and had above average G+C. Most supported the rRNA-based phylogeny, confirming A. tumefaciens to be the closest among these relatives, but 347 genes were incompatible with this phylogeny; these were scattered throughout the genome but were over-represented on the plasmids. An unexpectedly large number of genes were shared by all three rhizobia but were missing from A. tumefaciens. CONCLUSION: Overall, the genome can be considered to have two main components: a 'core', which is higher in G+C, is mostly chromosomal, is shared with related organisms, and has a consistent phylogeny; and an 'accessory' component, which is sporadic in distribution, lower in G+C, and located on the plasmids and chromosomal islands. The accessory genome has a different nucleotide composition from the core despite a long history of coexistence.
