(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Proteobacteria: NE > Alphaproteobacteria: NE > Rhizobiales: NE > Rhizobiaceae: NE > Rhizobium/Agrobacterium group: NE > Rhizobium: NE > Rhizobium tropici: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Rhizobium tropici CIAT 899: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MILKYAMSLAFACVLSTGLLMSGQARAEADDTKYDLGMIPAPHIMRPHGK VSNEVVLISDLAGWGDKEKAVADKLVANGSLVIGIDYPSFLAALDSKNDG CIYIVSDLESLSQQVQRSFADSTYQLPVIAGVGAGGAMALTIAAQTPDAT VAGTLAVDPTAGVGLKQELCTPAEKKTDGDMVFGLQEGALPNPILVTFTA NAPKDGRDHVADIQKDHPEVQTTDSDNADTYATLEASLADLMKTIDSSKS PLGLPLDIMETTPTEDTLAIVYSGDGGWRDIDKEVGSYLQDQGIPVVGVD TLHYFWTEKDPQQTANDLGRIIDFYTKRFKVKHVVLVGYSFGADVLPASY NRLPQAEKDKIVQMSLLSLSQKVDYVISVMGWLGASSQGKGGDPVNDLKS INPKMVQCVYGKDDDEDVACPLLKGTGAEVIAMDGGHHFDDDYEALANHI INGLKSRLGE
Rhizobium tropici CIAT899 is highly acid tolerant and a good competitor for Phaseolus vulgaris nodule occupancy at low pH values. Using Tn5 mutagenesis, we identified an operon required for acid tolerance and nodulation competitiveness. The insertion was mapped to the 5' end of atvA, encoding a product with high sequence identity to the agro-bacterial AcvB virulence protein. Complementation analyses indicated that atvA is an ortholog of acvB, both genes being required for acid tolerance. A Ser/Ala substitution in the LIPASE_SER motif of AtvA resulted in an acid sensitive Fix+ but very poorly competing strain, demonstrating that Ser-313 is essential for AtvA function. atvA is the second gene in an operon that is transcriptionally upregulated by acid shock. The acid-responsive promoter was mapped to a 469-bp intergenic region located upstream of lpiA, the first gene in the operon. lpiA-like genes are found in several alpha, beta, and gamma Proteobacteria that interact with eukaryotic host cells, and they are predicted to encode membrane proteins related to the FmtC/MprF family from low G+C Firmicutes. The latter proteins are involved in resistance to cationic antimicrobial peptides. A nonpolar deletion in lpiA caused a sevenfold decrease in relative nodulation competitiveness.
