(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Basidiomycota: NE > Pucciniomycotina: NE > Microbotryomycetes: NE > Sporidiobolales: NE > Sporidiobolaceae: NE > Rhodotorula: NE > Rhodotorula toruloides: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MLFPRFAVIAATAIGFAGSAKAAPVTAASLAVRATLCASPSGDAGTYSQP CDTYAQTINCPNGIRNATAGVVLLVHGTGSTGPETWDNGPYIQLLPNAGP GFDVCKITLPGRSLGDVQTTSEYVAFAIQQLAAQSQTGKVGIVSHSQGGL NVQWALDFWPSYRPLVSAFVALAGDFHGTGEGPPACLVLDATTGGCDPSV IQQSVGSNFLNALNSRGGVALVPTTSIYTIFDDVIQPELIPATSLLSGAT NVRLQDYCGPGYLVDHFVIPFASFAYAVAVDALTRQSTAQPDSVPKTSCA WLLDDVLLNNFQRGPTILREAVTDASAVITGPKVLEEPPLKPYVCARGDA TTGCA
Triacylglycerols are among the most attractive alternative raw materials for biofuel development. Current oil plant-based technologies are limited in terms of triacylglycerol production capacity and rate. These limitations may be circumvented by biotransformation of carbohydrates into lipids; however, our understanding of microbial oleaginicity remains limited. Here we present the results of a multi-omic analysis of Rhodosporidium toruloides, a robust triacylglycerol-producing fungus. The assembly of genome and transcriptome sequencing data reveals a genome of 20.2 Mb containing 8,171 protein-coding genes, the majority of which have multiple introns. Genes including a novel fatty acid synthase are predicted to participate in metabolic pathways absent in non-oleaginous yeasts. Transcriptomic and proteomic data suggest that lipid accumulation under nitrogen-limited conditions correlates with the induction of lipogenesis, nitrogenous compound recycling, macromolecule metabolism and autophagy. The multi-omic map of R. toruloides therefore provides a valuable resource for efforts to rationally engineer lipid-production pathways.
