(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Saccharomycetaceae: NE > Saccharomyces: NE > Saccharomyces paradoxus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MGVEEFSIHVSENELKDLKRRLSSARIPKNVERKNWDFGTNAEYLAEVIN YWKNSYDWRNIEQKLNGFHHFQTTISNIRIHYIHEKGKSANSIPIILTHG WPDSFLRYTKLIPLLTDPEKFGVSSGISFDVVIPSLPGFGFSDYPAGGSI NNDTISDIWLELMKNKLGYDRFLAAGGDIGSGVTRYLGFKYPQNLIGIHL TDVGIIRDLLNQSQLQSFSSEEQEYCKIASDWLDKEAGYMKIQSTKPQTL AFGLTDSPVGLAAWILEKFHSWRDLQTNLPLDDIITNIMIYWFGKNIYTA TRVYYENSHFLNPIGTIEVPTGICLFPKDILLPPRKWVEAHLNVVHWGNV QKGGHFTSMENPEEYAADLFLFLDKLG
Reference
Title: Fungal epoxide hydrolases: new landmarks in sequence-activity space Smit MS Ref: Trends Biotechnol, 22:123, 2004 : PubMed
Epoxide hydrolases are useful catalysts for the hydrolytic kinetic resolution of epoxides, which are sought after intermediates for the synthesis of enantiopure fine chemicals. The epoxide hydrolases from Aspergillus niger and from the basidiomycetous yeasts Rhodotorula glutinis and Rhodosporidium toruloides have demonstrated potential as versatile, user friendly biocatalysts for organic synthesis. A recombinant A. niger epoxide hydrolase, produced by an overproducing A. niger strain, is already commercially available and recombinant yeast epoxide hydrolases expressed in Escherichia coli have shown excellent results. Within the vast body of activity information on the one hand and gene sequence information on the other hand, the epoxide hydrolases from the Rhodotorula spp. and A. niger stand out because we have sequence information as well as activity information for both the wild-type and recombinant forms of these enzymes.