(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Holometabola: NE > Diptera: NE > Brachycera: NE > Muscomorpha: NE > Eremoneura: NE > Cyclorrhapha: NE > Schizophora: NE > Calyptratae: NE > Muscoidea: NE > Muscidae: NE > Muscinae: NE > Stomoxyini: NE > Stomoxys: NE > Stomoxys calcitrans: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MARSIKASLSSSSSSQSQSPLSSSSSRLSQSSSSLYSLKKSTSSSSLTSS LSRPSSFGLVSYLLATRNNDICRGLLATLVILLRMTALASAMTDHLIVQT TSGPVRGRSVTVQGREVHVFTGIPYAKPPVDDLRFRKPVPAEPWHGVLDA TRLPATCVQERYEYFPGFSGEEMWNPNTNVSEDCLFMNIWAPAKARLRHG RGTNGGEHSAKTDQDHLIHSATPQNTTNGLPILIWIYGGGFMTGSATLDI YNAEIMSAVGNVIVASFQYRVGAFGFLHLSPVMPGFEEEAPGNVGLWDQA LALRWLKENARAFGGNPEWMTLFGESAGSSSVNAQLMSPITRGLVKRGMM QSGTMNAPWSHMTSEKAVEIGKALINDCNCNASLLPDNPQAVMACMRSVD AKTISVQQWNSYSGILSLPSAPTIDGAFLPADPMTLLKTADLSGYDILIG NVKDEGTYFLLYDFIDYFDKDDATSLPRDKYLEIMNNIFNKATQAEREAI IFQYTSWEGNPGYQNQQQIGRAVGDHFFTCPTNEYAQSLAERGAGVHYYY FTHRTSTSLWGEWMGVLHGDEIEYFFGQPLNTSLQYRPVERELGKRMLNA VIEFAKTGNPAVDGEEWPNFSKEDPVYYVFSTDEKTEKLQRGPLAKRCSF WNDYLPKVRSWAGSECEGNISLSASGTIYQTQQQHSVLLNVALVLTLVFS IFQ
Reference
Title: Acetylcholinesterase of Stomoxys calcitrans (L.) (Diptera: Muscidae): cDNA sequence, baculovirus expression, and biochemical properties Temeyer KB, Chen AC Ref: Vet Parasitol, 184:92, 2012 : PubMed
A 2193-nucleotide cDNA encoding acetylcholinesterase (AChE) of the stable fly, Stomoxys calcitrans (L.) was sequenced and expressed in the baculovirus system. The open reading frame encoded a 91 amino acid secretion signal peptide and a 613 amino acid mature protein with 96% and 94% identity to the AChEs of Haematobia irritans (L.) and Musca domestica (L.), respectively. Structural characteristics of M. domestica, H. irritans, and Drosophila melanogaster AChEs were conserved in the S. calcitrans AChE. The recombinant enzyme was inhibited by eserine, coroxon, and paraoxon and exhibited K(m) values of 63.9muM for acetylthiocholine and 96.7muM for butyrylthiocholine, confirming its biochemical identity as an acetylcholinesterase (EC 3.1.1.7). These data will enable rapid identification and assay for mutations that reduce AChE sensitivity to organophosphate (OP) pesticides, potentially aiding resistance management efforts to prevent fixation of the mutations in pest populations.
