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Gene_locus Report for: strci-MONCII

Streptomyces cinnamonensis monensin cyclase

Comment
MonCII from the monensin biosynthetic gene cluster in Streptomyces cinnamonensis, which was previously thought to be an epoxide hydrolase, is a novel thioesterase. It hydrolyse several thioester substrates, including an N-acetylcysteamine thioester derivative of monensin A


Relationship
Family|Epoxide_hydrolase
Block| X
Position in NCBI Life Tree|Streptomyces cinnamonensis
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Actinobacteria [phylum]: N E > Actinobacteria [class]: N E > Streptomycetales: N E > Streptomycetaceae: N E > Streptomyces: N E > Streptomyces cinnamonensis: N E


Molecular evidence
Database
No mutation
No structure
No kinetic





No Substrate
No inhibitor
1 Genbank : AF440781
1 UniProt : Q846Y1
1 Interpro : Q846Y1
1 Prodom : Q846Y1
1 Pfam : Q846Y1
1 PIRSF : Q846Y1
1 SUPERFAM : Q846Y1
Sequence
Graphical view for this peptide sequence: strci-MONCII
Colored MSA for Epoxide_hydrolase (raw)
MKNLRIPVSQTVSLNVRYRPADGPGAPGRPFLLLHGMLSNARMWDEVAAR
LAAAGHPAYAVDHRGHGESDTPPDGYDNATVVTDLVAAVTALDLSGALVA
GHSWGAHLALRLAAEHPDLVAGLALIDGGWYEFDGPVMRAFWERTADVVR
RAQQGTTSAADMRAYLRATHPDWSPTSIEARLADYRVGPDGLLIPRLTST
QVMSIVAGLQREAPADWYPKVTVPVRLLPLIPAIPQLSDQVRAWVAAAEA
ALEQVSVRWYPGSDHDLHAGAPDEIAADLLLLARSCEAMPGGKAGVRPA
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MKNLRIPVSQTVSLNVRYRPADGPGAPGRPFLLLHGMLSNARMWDEVAAR
LAAAGHPAYAVDHRGHGESDTPPDGYDNATVVTDLVAAVTALDLSGALVA
GHSWGAHLALRLAAEHPDLVAGLALIDGGWYEFDGPVMRAFWERTADVVR
RAQQGTTSAADMRAYLRATHPDWSPTSIEARLADYRVGPDGLLIPRLTST
QVMSIVAGLQREAPADWYPKVTVPVRLLPLIPAIPQLSDQVRAWVAAAEA
ALEQVSVRWYPGSDHDLHAGAPDEIAADLLLLARSCEAMPGGKAGVRPA


References
    Title: Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin
    Harvey BM, Hong H, Jones MA, Hughes-Thomas ZA, Goss RM, Heathcote ML, Bolanos-Garcia VM, Kroutil W, Staunton J and Spencer JB <1 more author(s)>
    Ref: Chembiochem, 7:1435, 2006 : PubMed

            

    Title: Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization
    Oliynyk M, Stark CB, Bhatt A, Jones MA, Hughes-Thomas ZA, Wilkinson C, Oliynyk Z, Demydchuk Y, Staunton J, Leadlay PF
    Ref: Molecular Microbiology, 49:1179, 2003 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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