Other strains: TTC1275 strain hb27 / atcc baa-163 / dsm 7039 from Henne et al TTHA1638 strain HB8 from Masui et al. ; uncultured bacterium one aa diff found in structure
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Deinococcus-Thermus: NE > Deinococci: NE > Thermales: NE > Thermaceae: NE > Thermus: NE > Thermus thermophilus: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Thermus thermophilus HB27: N, E.
Thermus thermophilus HB8: N, E.
Thermus aquaticus (subsp. thermophilus): N, E.
Thermus thermophilus JL-18: N, E.
Thermus thermophilus SG0.5JP17-16: N, E.
uncultured bacterium: N, E.
uncultured bacteria: N, E.
Molecular evidence
Database
No mutation 1 structure: 1UFO: Crystal structure of TT1662 from Thermus thermophilus HB8 No kinetic
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRVRTERLTLAGLSVLARIPEAPKALLLALHGLQGSKEHILALLPGYAER GFLLLAFDAPRHGEREGPPPSSKSPRYVEEVYRVALGFKEEARRVAEEAE RRFGLPLFLAGGSLGAFVAHLLLAEGFRPRGVLAFIGSGFPMKLPQGQVV EDPGVLALYQAPPATRGEAYGGVPLLHLHGSRDLIVPLARMEKTLEALRP HYPEGRLARFVEEGAGHTLTPLMARVGLAFLEHWLEAR
References
Title: Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern Lu M, Schneider D, Daniel R Ref: Front Microbiol, 13:851969, 2022 : PubMed
Lipolytic enzymes are one of the most important enzyme types for application in various industrial processes. Despite the continuously increasing demand, only a small portion of the so far encountered lipolytic enzymes exhibit adequate stability and activities for biotechnological applications. To explore novel and/or extremophilic lipolytic enzymes, microbial consortia in two composts at thermophilic stage were analyzed using function-driven and sequence-based metagenomic approaches. Analysis of community composition by amplicon-based 16S rRNA genes and transcripts, and direct metagenome sequencing revealed that the communities of the compost samples were dominated by members of the phyla Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Chloroflexi. Function-driven screening of the metagenomic libraries constructed from the two samples yielded 115 unique lipolytic enzymes. The family assignment of these enzymes was conducted by analyzing the phylogenetic relationship and generation of a protein sequence similarity network according to an integrated classification system. The sequence-based screening was performed by using a newly developed database, containing a set of profile Hidden Markov models, highly sensitive and specific for detection of lipolytic enzymes. By comparing the lipolytic enzymes identified through both approaches, we demonstrated that the activity-directed complements sequence-based detection, and vice versa. The sequence-based comparative analysis of lipolytic genes regarding diversity, function and taxonomic origin derived from 175 metagenomes indicated significant differences between habitats. Analysis of the prevalent and distinct microbial groups providing the lipolytic genes revealed characteristic patterns and groups driven by ecological factors. The here presented data suggests that the diversity and distribution of lipolytic genes in metagenomes of various habitats are largely constrained by ecological factors.
Thermus thermophilus HB27 is an extremely thermophilic, halotolerant bacterium, which was originally isolated from a natural thermal environment in Japan. This organism has considerable biotechnological potential; many thermostable proteins isolated from members of the genus Thermus are indispensable in research and in industrial applications. We present here the complete genome sequence of T. thermophilus HB27, the first for the genus Thermus. The genome consists of a 1,894,877 base pair chromosome and a 232,605 base pair megaplasmid, designated pTT27. The 2,218 identified putative genes were compared to those of the closest relative sequenced so far, the mesophilic bacterium Deinococcus radiodurans. Both organisms share a similar set of proteins, although their genomes lack extensive synteny. Many new genes of potential interest for biotechnological applications were found in T. thermophilus HB27. Candidates include various proteases and key enzymes of other fundamental biological processes such as DNA replication, DNA repair and RNA maturation.
Thermus thermophilus TT1662 TTC1275 TTHA1638 (strain HB27/ATCC BAA-163/DSM 7039 and HB8); uncultured bacterium
