(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Euglenozoa: NE > Kinetoplastida: NE > Trypanosomatidae: NE > Trypanosoma: NE > Trypanozoon: NE > Trypanosoma brucei: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Trypanosoma brucei TREU927: N, E.
Trypanosoma brucei gambiense: N, E.
Trypanosoma brucei brucei strain 927/4 GUTat10.1: N, E.
Trypanosoma brucei brucei: N, E.
Trypanosoma brucei gambiense DAL972: N, E.
Trypanosoma brucei brucei TREU927: N, E.
Trypanosoma brucei equiperdum: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MPTLPLSKYIDELKALEGLLAVDGKPRMLKFVGDQLYWLQGMDQELYSVN IADHNATPSRVIDRCRKGCTAAEGKEDSTVETKEEELLRERLRSRVTGIS SYRVRCRDGADFFTSGANIFLYRPASGKPPLKVLDYIGDEELKQRFSSEK KPFLCIEYFSHLANDAGACDSTKDRADVISFVYSNNLYIATVVEGSGEAS HSREPPLRVNVECVTTFGDELHSCGDADYIIQEEFGRYTGHYATAEYVLF SYTDVSMLREVMLIDGSTDSGVEKMPYCRVGDPNARTVLVVYERRTKRYR VVPSSAIAAIAPWAEYMPRFGFKDEHTIYFSVLSRTQEKMSLLSCRINSL PVVDAEALASCFSEAARDTDVAAVAAGTTLATRAATVNLTTEWHQHIPWA WVDVQPGVALHLCGKYEVLVRHANESESAHSHVYARAQGGDEGSWRPLTC GAWNVRAGGLIVKDDRVFFLANARNRLESVLYSVVINLDAGPVSAEELTQ LTPSGEHVYSFAVLNNTLCYVSSTATTPAELHISSINNPGSRWVVPTRHL WDDTSEKAVTPRTTLEGLTVPHIINIVNRRGVPLSGRLFVSPHAALGKLN PLAMYIYGGPHAQLVYDSDFDGACKPIFQMLASFGISVLVADGQMSNANG LRDHSICKHSMGSFETSDYVDWVRHMTNCKELPCGFCADPERVAIFGWSY GGYATLLAMSQAPDVFKIGFAGAPVGDWTLYDTGYTERYMGLLHTNADRT SGSKGDATAKCAISDAYTRSAIKAFASNFPEELNRVFIAHGLLDENVHFC HTSVVVNAMIDAGKPCCSVVYPGERHSLRKKKASRLHHDAMLAKTLMEVL
BACKGROUND: Trypanosoma brucei gambiense is the causative agent of chronic Human African Trypanosomiasis or sleeping sickness, a disease endemic across often poor and rural areas of Western and Central Africa. We have previously published the genome sequence of a T. b. brucei isolate, and have now employed a comparative genomics approach to understand the scale of genomic variation between T. b. gambiense and the reference genome. We sought to identify features that were uniquely associated with T. b. gambiense and its ability to infect humans. METHODS AND FINDINGS: An improved high-quality draft genome sequence for the group 1 T. b. gambiense DAL 972 isolate was produced using a whole-genome shotgun strategy. Comparison with T. b. brucei showed that sequence identity averages 99.2% in coding regions, and gene order is largely collinear. However, variation associated with segmental duplications and tandem gene arrays suggests some reduction of functional repertoire in T. b. gambiense DAL 972. A comparison of the variant surface glycoproteins (VSG) in T. b. brucei with all T. b. gambiense sequence reads showed that the essential structural repertoire of VSG domains is conserved across T. brucei. CONCLUSIONS: This study provides the first estimate of intraspecific genomic variation within T. brucei, and so has important consequences for future population genomics studies. We have shown that the T. b. gambiense genome corresponds closely with the reference, which should therefore be an effective scaffold for any T. brucei genome sequence data. As VSG repertoire is also well conserved, it may be feasible to describe the total diversity of variant antigens. While we describe several as yet uncharacterized gene families with predicted cell surface roles that were expanded in number in T. b. brucei, no T. b. gambiense-specific gene was identified outside of the subtelomeres that could explain the ability to infect humans.
African trypanosomes cause human sleeping sickness and livestock trypanosomiasis in sub-Saharan Africa. We present the sequence and analysis of the 11 megabase-sized chromosomes of Trypanosoma brucei. The 26-megabase genome contains 9068 predicted genes, including approximately 900 pseudogenes and approximately 1700 T. brucei-specific genes. Large subtelomeric arrays contain an archive of 806 variant surface glycoprotein (VSG) genes used by the parasite to evade the mammalian immune system. Most VSG genes are pseudogenes, which may be used to generate expressed mosaic genes by ectopic recombination. Comparisons of the cytoskeleton and endocytic trafficking systems with those of humans and other eukaryotic organisms reveal major differences. A comparison of metabolic pathways encoded by the genomes of T. brucei, T. cruzi, and Leishmania major reveals the least overall metabolic capability in T. brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified.
Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.
Trypanosoma brucei hypothetical protein