Xanthobacter agilis. o-phthalyl amidase
Phthalimido is a stable protecting group in beta-lactam chemistry but no conventional chemical methods effectively removes this group. xanag-pham catalyzes the removal of the phthalyl group from phthalyl amides generating phthalate and an amine. The enzyme has a broad substrate specificity and hydrolyzes phthalylated amino acids, peptides, beta-lactams, aromatic and aliphatic amines\; substitutions allowed on the phthalyl group include 6-F, 6-NH2, 3-OH, and a nitrogen in the aromatic ring ortho to the carboxy group attached to the amine.
MQAPSVHQHV AFTEEIGDLP DGSSYMIRVP ENWNGVLIRD LDLVSGTSNS NAARYETMLK EGFAVAGTAR HPLRQWQYDP AHEIENLNHV LDTFEENYGS PERVIQYGCS GGAHVSLAVA EDFSDRVDGS VALAAHTPVW IMNSFLDGWF SLQSLIGEYY VEAGHGPLSD LAITKLPNDG SSNSSGHGME GDLPAAWRNA FTAANATPEG RARMALAFAL GQWSPWLADN TPQPDLDDPE AIADSVYESA MRLAGSPGGE ARIMFENAAR GQQLSWNDDI DYADFWENSN PAMKSAVQEL YDTAGLDLQS DIETVNSQPR IEASQYALDY WNTPGRNVIG DPEVPVLRLH MIGDYQIPYS LVQGYSDLIS ENNNDDLYRT AFVQSTGHCN FTAAESSAAI EVMMQRLDTG EWPSTEPDDL NAIAEASNTG TEARFMALDG WEIPEYNRTW KPE
| Title : Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis - Briggs_1996_J.Mol.Catal.B.Enzym_2_53 |
| Author(s) : Briggs BS , Kreuzman AJ , Whitesitt C , Yeh WK , Zmijewski M |
| Ref : J Mol Catal B Enzym , 2 :53 , 1996 |
| Abstract : Briggs_1996_J.Mol.Catal.B.Enzym_2_53 |
| ESTHER : Briggs_1996_J.Mol.Catal.B.Enzym_2_53 |
| PubMedSearch : Briggs_1996_J.Mol.Catal.B.Enzym_2_53 |
| PubMedID: |
| Gene_locus related to this paper: xanag-pham |
| Title : o-Phthalyl amidase in the synthesis of loracarbef: Process development using this novel biocatalyst - Black_1996_Biotechnol.Lett_18_875 |
| Author(s) : Black TD , Briggs BS , Evans R , Muth WL , Vangala S , Zmijewski M |
| Ref : Biotechnol Lett , 18 :875 , 1996 |
| Abstract : Black_1996_Biotechnol.Lett_18_875 |
| ESTHER : Black_1996_Biotechnol.Lett_18_875 |
| PubMedSearch : Black_1996_Biotechnol.Lett_18_875 |
| PubMedID: |
| Gene_locus related to this paper: xanag-pham |