Mutation

Kinetic parameters

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Kinetic parameters for mutation WT_eleel-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
2-Aminoethylacetate 24000 & 3000 uM 114000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-Bromoethylacetate 9900 & 600 uM 57600/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-Chloroethylacetate 32000 & 2000 uM 90000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-Cyanoethylacetate 17000 & 2000 uM 66000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-Hydroxyethylacetate 22000 & 4000 uM 9600/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-Methoxyethylacetate 4700 & 700 uM 49800/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-N,N-dimethylaminoethylacetate 1100 & 100 uM 288000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
2-N-methylaminoethylacetate 20000 & 1000 uM 132000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
3,3-dimethylbutylacetate 2600 & 300 uM 186000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
3-methyl-1butylacetate 2600 & 700 uM 72000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
Acetylcholine 330 & 70 uM 960000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
Acetylcholine 100 uM 960000 /min   Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834
Acetylthiocholine 130 uM 900000 /min   Salih_1992_Biochem.J_285_451
Acetylthiocholine - 733333/min 100mM NaP T28C pH7.0 Vigny_1978_Eur.J.Biochem_85_317
Ethylacetate 220000 & 29000 uM 22200/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
Methyl-butyrate 33000 & 5000 uM 15600/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
Methyl-hexanoate 12000 & 2000 uM 78000/min 0.18MNaCl pH 7.8 T25C Hasan_1980_J.Biol.Chem_255_3898
Paranitrophenylacetate 5000 uM 76800 /min   Rosenberry_1975_Proc.Natl.Acad.Sci.U.S.A_72_3834
Paranitrophenylacetate 4900 uM 78000 /min   Salih_1992_Biochem.J_285_451

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
MEPQ 5.3 10-8/M/min - -   Levy_1986_Biochem.Pharmacol_35_1079

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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