Mutation
Kinetic parameters
Tree Display
AceDB Schema
XML Display
Feedback

Kinetic parameters for mutation D131N_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
3,3-dimethylbutylthioacetate 300 & 50 uM 55000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
Acetylthiocholine 140 & 10 uM 390000 & 40000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
Edrophonium 2.1 & 0.3 uM - - 50mM NaPhosphate pH8 3.3-dimethylbutylthioacetate Kronman_1994_J.Biol.Chem_269_27819
Edrophonium 1.0 & 0.2 uM - - 50mM NaPhosphate pH8 Acetylthiocholine Kronman_1994_J.Biol.Chem_269_27819
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer