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Kinetic parameters for mutation D74G_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 630 & 200 uM 360000 & 10000/min pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 - - 4000 nM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Decamethonium - - 850 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Edrophonium - - 7.5 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Propidium - - 90 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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