Mutation
Kinetic parameters
Tree Display
AceDB Schema
XML Display
Feedback

Kinetic parameters for mutation D74N_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 600 uM 250000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
Acetylthiocholine 580 & 200 uM 270000 & 11000/min pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 2450 nM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
BW284C51 - - 5650 nM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Decamethonium 850 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Decamethonium - - 850 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Edrophonium 3.5 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Edrophonium - - 10.5 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Hexamethonium 2.0 mM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
HuperzineA 17 & 2 nM 25 & 5 nM - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95
Propidium 7.8 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Propidium - - 6.15 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Tacrine 4600 & 700 nM 65000 & 15000 nM - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer