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Kinetic parameters for mutation F295A_mouse-ACHE
Substrate
Molecule
Km
Kcat
Exp. conditions
Reference
Acetylthiocholine
64 uM
73000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
Benzoylthiocholine
16 uM
390 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
Butyrylthiocholine
3.6 uM
8000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
Inhibitor
Molecule
Ki
Ksi
Ic50
Exp. conditions
Substrate
Reference
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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