Mutation
Kinetic parameters
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Kinetic parameters for mutation M70Y/K285D_bunfa-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 95.3 & 5.9 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 3.8 & 0.2 nM 9.3 & 0.1 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
D-tubocurarine 11.8 & 1.6 uM 26.5 & 2.7 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Decamethonium 1.3 & 0.1 uM 4.4 & 0.7 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Edrophonium 0.6 & 0.0 uM 7.8 & 2.0 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Gallamine~Flaxedil 8.0 & 1.1 uM - - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Propidium 0.6 & 0.1 uM 1.0 & 0.2 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
WT-fasciculin2 - 0.3 & 0.2 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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