Mutation

Kinetic parameters

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Kinetic parameters for mutation M70Y_bunfa-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 108.6 & 4.5 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 7.9 & 1.3 nM 17.5 & 1.4 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
D-tubocurarine 92.4 & 13.2 uM 266.0 & 86.0 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Decamethonium 3.8 & 0.4 uM 10.2 & 2.5 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Edrophonium 0.6 & 0.0 uM 9.6 & 0.0 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Gallamine~Flaxedil 222.0 & 23.8 uM - - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Propidium 1.6 & 0.0 uM 2.3 & 0.4 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
WT-fasciculin2 - 5.6 & 1.3 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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