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Kinetic parameters for mutation W286A_human-ACHE
Substrate
Molecule
Km
Kcat
Exp. conditions
Reference
3,3-dimethylbutylthioacetate
400 uM
46000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
Acetylthiocholine
200 uM
320000/min
50mM Na phosphate pH8
Barak_1994_J.Biol.Chem_269_6296
Acetylthiocholine
250 uM
4.1 10+5/min
IS 50mM phosphate pH8 T27C
Kaplan_2001_Biochemistry_40_7433
Acetylthiocholine
400 uM
400000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
Acetylthiocholine
280 & 60 uM
400000 & 10000/min
pH8 T27C 50mM Na phosphate
Shafferman_1992_EMBO.J_11_3561
Butyrylthiocholine
0.20 mM
0.27 10+5/min
IS 50mM phosphate pH8 T27C
Kaplan_2001_Biochemistry_40_7433
Paranitrophenylacetate
4600 uM
130000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
S-N-Propylthioacetate
8200 uM
9600 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
Inhibitor
Molecule
Ki
Ksi
Ic50
Exp. conditions
Substrate
Reference
BW284C51
121 nM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
BW284C51
-
-
650 nM
pH8 T27C 50mM Na phosphate
Acetylthiocholine
Shafferman_1992_EMBO.J_11_3561
Decamethonium
41 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Decamethonium
-
-
76.5 uM
pH8 T27C 50mM Na phosphate
Acetylthiocholine
Shafferman_1992_EMBO.J_11_3561
Edrophonium
0.6 uM
-
-
3-dimethylbutylthioacetate
3
IS 50mM phosphate pH8 T27C
Edrophonium
0.6 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Edrophonium
0.7 uM
-
-
IS 50mM phosphate pH8 T27C
Acetylthiocholine
Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium
-
-
2.25 uM
pH8 T27C 50mM Na phosphate
Acetylthiocholine
Shafferman_1992_EMBO.J_11_3561
Edrophonium
0.6 uM
-
-
IS 50mM phosphate pH8 T27C
Paranitrophenylacetate
Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium
0.3 uM
-
-
IS 50mM phosphate pH8 T27C
S-N-Propylthioacetate
Ordentlich_1993_J.Biol.Chem_268_17083
Hexamethonium
0.3 mM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
HuperzineA
74 nM
-
-
50mM Na phosphate pH8 T22C
Acetylthiocholine
Ariel_1998_Biochem.J_335_95
Propidium
14.3 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Propidium
-
-
18.0 uM
pH8 T27C 50mM Na phosphate
Acetylthiocholine
Shafferman_1992_EMBO.J_11_3561
Tacrine
80 & 10 nM
150 & 20 nM
-
50mM Na phosphate pH8 T22C
Acetylthiocholine
Ariel_1998_Biochem.J_335_95
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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