Mutation

Kinetic parameters

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Kinetic parameters for mutation Y72A/W286A_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 300 uM 420000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 1290 nM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Decamethonium 81 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Edrophonium 0.6 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Hexamethonium 1.0 mM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Propidium 103 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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