Mutation
Kinetic parameters
Tree Display
AceDB Schema
XML Display
Feedback

Kinetic parameters for mutation Y72N/Y124Q_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 140 mM 4.1 10+5/min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
Butyrylthiocholine 0.25 mM 0.20 10+5/min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
Decamethonium 200 uM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
Edrophonium 1.1 uM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
HuperzineA 90 nM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
Tacrine 55 nM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer