Mutation

Kinetic parameters

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Kinetic parameters for mutation Y72N/D74N/Y124Q/W286A_mouse-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 2100 & 300 uM 150000 & 10000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 170000 & 6000 nM 690000 & 18000 nM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Decamethonium 16000 & 1000 uM 55000 & 35000 uM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Edrophonium 17 & 3 uM >120 uM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Propidium 1100 & 430 uM 2700 & 1200 uM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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