Mutation

Kinetic parameters

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Kinetic parameters for mutation Y72N/D74N/Y124Q/W286R_mouse-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 6100 & 730 uM 140000 & 20000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 780000 & 28000 nM 1000000 & 240000 nM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Decamethonium 50000 & 18000 uM 40000 & 35000 uM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Edrophonium 28 & 6 uM - - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074
Propidium 1900 & 760 uM 3200 & 1600 uM - 100mM NaP pH7.0 T22C eq3.gif sch3.gif Acetylthiocholine Radic_1993_Biochemistry_32_12074

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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