Torpedo_number
Kinetic parameters
Tree Display
AceDB Schema
XML Display
Feedback

Kinetic parameters for torpedo number 128 for human-ACHE

Substrates: 3,3-dimethylbutylthioacetate, Acetylthiocholine

Inhibitors: Edrophonium

Substrates

Substrate: 3,3-dimethylbutylthioacetate
EnzymeKmKcatExp. conditionsReference
D131N 300 & 50 uM 55000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 300 & 6 mM 55000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 450 uM 52000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 300 uM 55000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT 260 & 30 uM 46000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448

Substrate: Acetylthiocholine
EnzymeKmKcatExp. conditionsReference
D131N 140 & 10 uM 390000 & 40000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 120 uM 420000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
WT 140 uM 4.0 10+5 /min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
WT 140 & 10 uM 400000 & 50000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 140 uM 370000/min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 140 uM 400000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT 140 & 50 uM 370000 & 10000/min pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561
WT 80 & 10 uM 400000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 117 uM 390000 /min 50mM Na phosphate pH8.0 T27C Velan_1991_J.Biol.Chem_266_23977
WT 0.30 mM - IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
WT 2.5 mM - 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423


Inhibitors

Inhibitor: Edrophonium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
D131N 2.1 & 0.3 uM - - 3.3-dimethylbutylthioacetate 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
D131N 1.0 & 0.2 uM - - Acetylthiocholine 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 0.6 & 0.1 uM - - 3.3-dimethylbutylthioacetate 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 0.75 uM - - Acetylthiocholine 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
WT 50 uM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433
WT 0.7 & 0.1 uM - - Acetylthiocholine 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 0.3 uM - - Acetylthiocholine IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 0.6 uM 4.8 uM - Acetylthiocholine 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT - - 1.5 uM Acetylthiocholine pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561
WT 0.32 & 0.05 uM - - Acetylthiocholine IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 0.4 uM - - Paranitrophenylacetate IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 0.24 uM - - S-N-Propylthioacetate IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer