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Kinetic parameters for torpedo number 288 for human-BCHE

Substrates: Butyrylthiocholine

Inhibitors: DFP, Echothiophate, Iso-OMPA

Substrates

Substrate: Butyrylthiocholine
EnzymeKmKcatExp. conditionsReference
L286D 8.8 mM - 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286K 22 mM - 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286Q 19.8 mM - 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286R 11 mM - 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
WT 7 & 0.7 uM 24000 & 10000 /min 100mM K phosphate pH7.0 T25C Blong_1997_Biochem.J_327_747
WT 2.2 mM - 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
WT 0.05 mM 1.1 10+5 /min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
WT 2.8 & 0.7 mM 98000 & 33000 /min 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423


Inhibitors

Inhibitor: DFP
EnzymeKiKsiIc50SubstrateExp. conditionsReference
L286D - - 0.17 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286Q - - 0.25 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
WT - - 0.02 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749

Inhibitor: Echothiophate
EnzymeKiKsiIc50SubstrateExp. conditionsReference
L286D - - 2.5 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286K - - >1000 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286Q - - 5.5 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
WT - - 0.27 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749

Inhibitor: Iso-OMPA
EnzymeKiKsiIc50SubstrateExp. conditionsReference
L286D - - 55 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286K - - 100 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
L286Q - - 140 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
WT - - 22 uM Butyrylthiocholine 100mM Na phosphate pH7.4 T22C Gnatt_1994_J.Neurochem_62_749
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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