Torpedo_number

Kinetic parameters

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Kinetic parameters for torpedo number 443

Substrates:

Inhibitors: HuperzineA, Tacrine

Substrates



Inhibitors

Inhibitor: HuperzineA
Gene_LocusEnzymeKiKsiIc50SubstrateExp. conditionsReference
human-ACHEE450A 6 & 1 nM - - Acetylthiocholine 50mM Na phosphate pH8 T22C Ariel_1998_Biochem.J_335_95
human-ACHEWT 16 & 1 nM 12 & 4 nM - Acetylthiocholine 50mM Na phosphate pH8 T22C Ariel_1998_Biochem.J_335_95
human-ACHEWT 16 nM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433
human-BCHEWT 50000 nM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433

Inhibitor: Tacrine
Gene_LocusEnzymeKiKsiIc50SubstrateExp. conditionsReference
human-ACHEE450A 530 & 50 nM 870 & 70 nM - Acetylthiocholine 50mM Na phosphate pH8 T22C Ariel_1998_Biochem.J_335_95
human-ACHEWT 85 & 12 nM 90 & 20 nM - Acetylthiocholine 50mM Na phosphate pH8 T22C Ariel_1998_Biochem.J_335_95
human-ACHEWT 12 nM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433
human-ACHEWT 0.21 uM - - Acetylthiocholine 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
human-BCHEWT 12 nM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433
mouse-ACHEWT 40 & 10 nM 130 & 50 nM - Acetylthiocholine 100mM NaP pH7.0 T22C Radic_1993_Biochemistry_32_12074
mouse-BCHEWT 17 & 4 nM 47 & 22 nM - Acetylthiocholine 100mM NaP pH7.0 T22C Radic_1993_Biochemistry_32_12074
human-BCHEWT 0.06 & 0.03 uM - - Butyrylthiocholine 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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