Torpedo_number

Kinetic parameters

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Kinetic parameters for torpedo number 70 for bunfa-ACHE

Substrates: Acetylthiocholine

Inhibitors: BW284C51, D-tubocurarine, Decamethonium, Edrophonium, Gallamine~Flaxedil, Propidium, WT-fasciculin2

Substrates

Substrate: Acetylthiocholine
EnzymeKmKcatExp. conditionsReference
M70Y/K285D 95.3 & 5.9 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 108.6 & 4.5 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 78.8 & 6.3 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099


Inhibitors

Inhibitor: BW284C51
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 3.8 & 0.2 nM 9.3 & 0.1 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 7.9 & 1.3 nM 17.5 & 1.4 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 16.9 & 1.6 nM 28.9 & 0.8 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: D-tubocurarine
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 11.8 & 1.6 uM 26.5 & 2.7 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 92.4 & 13.2 uM 266.0 & 86.0 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 39.1 & 2.8 uM 66.4 & 11.5 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: Decamethonium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 1.3 & 0.1 uM 4.4 & 0.7 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 3.8 & 0.4 uM 10.2 & 2.5 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 8.2 & 1.2 uM 47.5 & 3.5 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: Edrophonium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 0.6 & 0.0 uM 7.8 & 2.0 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 0.6 & 0.0 uM 9.6 & 0.0 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 0.6 & 0.0 uM 9.9 & 4.2 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: Gallamine~Flaxedil
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 8.0 & 1.1 uM - - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 222.0 & 23.8 uM - - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 512.0 & 58.7 uM - - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: Propidium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D 0.6 & 0.1 uM 1.0 & 0.2 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y 1.6 & 0.0 uM 2.3 & 0.4 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT 46.0 & 3.6 uM 250.0 & 60.0 uM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor: WT-fasciculin2
EnzymeKiKsiIc50SubstrateExp. conditionsReference
M70Y/K285D - 0.3 & 0.2 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
M70Y - 5.6 & 1.3 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099
WT - 50.7 & 2.6 nM - Acetylthiocholine T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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