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Kinetic parameters for torpedo number 70,121 for mouse-ACHE

Substrates: Acetylthiocholine

Inhibitors: BW284C51, Decamethonium, Propidium, WT-fasciculin2

Substrates

Substrate: Acetylthiocholine
EnzymeKmKcatExp. conditionsReference
Y72N/Y124Q 95 & 11 uM 94000 & 20000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074
WT 46 uM 140000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
WT 46 & 3 uM 140000 & 10000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074


Inhibitors

Inhibitor: BW284C51
EnzymeKiKsiIc50SubstrateExp. conditionsReference
Y72N/Y124Q 190 & 80 nM 460 & 130 nM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074
WT 2.8 & 1.0 nM 4.8 & 1.8 nM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074

Inhibitor: Decamethonium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
Y72N/Y124Q 130 & 14 uM 510 & 180 uM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074
WT 3.5 & 1.6 uM 31 & 16 uM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074

Inhibitor: Propidium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
Y72N/Y124Q 35 & 7 uM 120 & 44 uM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074
WT 2.2 & 0.7 uM 5.3 & 1.8 uM - Acetylthiocholine 100mM NaP pH7.0 T22C eq3.gif sch3.gif Radic_1993_Biochemistry_32_12074

Inhibitor: WT-fasciculin2
EnzymeKiKsiIc50SubstrateExp. conditionsReference
Y72N/Y124Q - 95 & 10 nM - Acetylthiocholine 100mM NaP pH7.0 T37C eq4 sch4 meth6 Radic_1994_J.Biol.Chem_269_11233
WT - 2.3 & 0.6 pM - Acetylthiocholine 50mM NaP pH8.0 Marchot_1997_J.Biol.Chem_272_3502
WT - 6.3 pM - Acetylthiocholine 100mM NaP pH7.0 T37C eq4 sch4 meth5 Radic_1994_J.Biol.Chem_269_11233
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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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