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Mutation Report for: A199S/F227A/S287G/A328W/Y332G/N535C_human-BCHE

A199S/F227A/S287G/A328W/Y332G/N535C_human-BCHE
Gene_Locus|human-BCHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|A199S/F227A/S287G/A328W/Y332G/N535C
Torpedo number|201//229//289//330//334//537//201,229,289,330,334,537
Summary|
Comment|p.A199S/F227A/S287G/A328W/Y332G/N535C Ala199Ser/Phe227Ala/Ser287Gly/Ala328Trp/Tyr332Gly/Asn535Cys (p.A227S/F255A/S315G/A356W/Y360G/N563C Ala227Ser/Phe255Ala/Ser315Gly/Ala356Trp/Tyr360Gly/Asn563Cys in primary sequence with 28 amino-acids signal peptide) denoted E377-516 for convenience. Mutation N535C introduces a new disulfide cross-subunit bond on the BChE mutated for increased cocaine hydrolysis
Kinetic parameters|none


References:
    Title: Amino-acid mutations to extend the biological half-life of a therapeutically valuable mutant of human butyrylcholinesterase
    Fang L, Hou S, Xue L, Zheng F, Zhan CG
    Ref: Chemico-Biological Interactions, 214C:18, 2014 : PubMed

            




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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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