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Mutation Report for: A199S/F227A/S287G/A328W/Y332G_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|A199S/F227A/S287G/A328W/Y332G
Torpedo number|201//229//289//330//334//201,229,289,330,334
Comment|p.A199S/F227A/S287G/A328W/Y332G Ala199Ser/Phe227Ala/Ser287Gly/Ala328Trp/Tyr332Gly (p.A227S/F255A/S315G/A356W/Y360G Ala227Ser/Phe255Ala/Ser315Gly/Ala356Trp/Tyr360Gly in primary sequence with 28 amino-acids signal peptide) denoted E12-7 or CocH3 for convenience Cocaine hydrolysis; 2000-fold improved catalytic efficiency against-cocaine E9 . The plant expressed enzyme called PCocSH
Kinetic parameters|none

    Title: A plant-derived cocaine hydrolase prevents cocaine overdose lethality and attenuates cocaine-induced drug seeking behavior
    Larrimore KE, Kannan L, Kendle RP, Jamal T, Barcus M, Stefanko K, Kilbourne J, Brimijoin S, Zhan CG and Mor TS <1 more author(s)>
    Ref: Prog Neuropsychopharmacol Biological Psychiatry, :109961, 2020 : PubMed


    Title: Development of a long-acting Fc-fused cocaine hydrolase with improved yield of protein expression
    Chen X, Deng J, Zheng X, Zhang J, Zhou Z, Wei H, Zhan CG, Zheng F
    Ref: Chemico-Biological Interactions, 306:89, 2019 : PubMed


    Title: Plasma butyrylcholinesterase regulates ghrelin to control aggression
    Chen VP, Gao Y, Geng L, Parks RJ, Pang YP, Brimijoin S
    Ref: Proc Natl Acad Sci U S A, 112:2251, 2015 : PubMed


    Title: Amino-acid mutations to extend the biological half-life of a therapeutically valuable mutant of human butyrylcholinesterase
    Fang L, Hou S, Xue L, Zheng F, Zhan CG
    Ref: Chemico-Biological Interactions, 214C:18, 2014 : PubMed


    Title: Kinetic characterization of high-activity mutants of human butyrylcholinesterase for the cocaine metabolite norcocaine
    Zhan M, Hou S, Zhan CG, Zheng F
    Ref: Biochemical Journal, 457:197, 2014 : PubMed


    Title: Design of high-activity mutants of human butyrylcholinesterase against (-)-cocaine: structural and energetic factors affecting the catalytic efficiency
    Zheng F, Yang W, Xue L, Hou S, Liu J, Zhan CG
    Ref: Biochemistry, 49:9113, 2010 : PubMed


    Title: Most efficient cocaine hydrolase designed by virtual screening of transition states
    Zheng F, Yang W, Ko MC, Liu J, Cho H, Gao D, Tong M, Tai HH, Woods JH, Zhan CG
    Ref: Journal of the American Chemical Society, 130:12148, 2008 : PubMed


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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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