E202A_human-ACHE

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Catalysis Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097 Shafferman_1995_5th.ChE.Meeting.Madras__189 Shafferman_1996_Biochem.J_318_833 || H-bond network Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097 Shafferman_1995_5th.ChE.Meeting.Madras__189 Shafferman_1996_Biochem.J_318_833 || Substrate inhibition E202 mutant not inhibited at high substrate concentration Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097 Shafferman_1995_5th.ChE.Meeting.Madras__189 Shafferman_1996_Biochem.J_318_833 || Acylation,Phosphorylation low effect on inhibition by DFP and DEFP but high for Paraoxon Ordentlich_1995_5th.ChE.Meeting.Madras__221 Ordentlich_1996_J.Biol.Chem_271_11953 Shafferman_1996_Biochem.J_318_833 || Aging E202 contributes to the aging process by stabilizing the imidazolium His447 Shafferman_1996_Biochem.J_318_833

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Acylation,Phosphorylation || Substrate inhibition || Aging || H-bond network

Torpedo_number : 199

Kinetic Parameter : BW284C51_E202A_human-ACHE, Decamethonium_E202A_human-ACHE, Propidium_E202A_human-ACHE, Edrophonium_E202A_human-ACHE, Acetylthiocholine_E202A_human-ACHE, Tacrine_E202A_human-ACHE, HuperzineA_E202A_human-ACHE

News : No news

Comment : p.E202A Glu202Ala (p.E233A Glu233Ala in primary sequence with 31 amino-acids signal peptide) Catalysis\;Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation\; H-bond network\; not inhibited at high substrate concentration\; Acylation,Phosphorylation\;low effect on inhibition by DFP and DEFP but high for Paraoxon\; Aging:E202 contributes to the aging process by stabilizing the imidazolium His447