Chebchoub_2024_Nat.Prod.Res__1

The polyphenolic compounds of the n-butanol fraction of Linum tenue Desf. (BFLTe) were characterised by RP-UHPLC-ESI-QTOF-MS analyses with the main presence of 6,8-di-C-glucosyl naringenin (11.7%), vicenin 2-isomer 2 (8.18%), luteolin-7,3'-di-O-beta-D-glucoside (7.18%), isovitexin (5.98%), luteolin-7-O-beta-D-glucoside (5.713%), myricitrin (4.41%), luteolin-4'-O-beta-D-glucoside (4.04%), chlorogenic acid (28.68%), 3-(2,6-dihydroxyphenyl)-4-hydroxy-6-methyl-3H-2-benzofuran-1-one (8.17%) and p-coumaric acid (4.0%.). The antioxidant capacity was evaluated using three complementary methods (DPPH, ABTS and Reducing power). Additionally, the antimicrobial activity was tested against eight bacterial strains and the fungi Candida albicans whereas the antidiabetic activity was performed against alpha-amylase. The anti-Alzheimer activity was tested by inhibiting the butyrylcholinesterase (BChE). The BFLTe showed, for the first-time, a good antioxidant potential in DPPH (IC(50):68.83 +/- 2.74 microg/mL), ABTS (IC(50):48.73 +/- 1.07 microg/mL) and Reducing power assays (A(0.50):99.98 +/- 1.18 microg/mL) and a moderate antimicrobial activity with 250 and 500 microg/mL MICs values. Moreover, the fraction exhibited an excellent inhibition of the BChE (IC(50):33.00 +/- 0.85 microg/mL) and alpha-amylase (IC(50):1093.13 +/- 12.93 microg/mL).