| Title : Carboxylesterase-activated hepatocyte-targeting fluorescent probe for drug-induced liver injury diagnosis - Liu_2025_Bioorg.Chem_162_108587 |
| Author(s) : Liu M , Ding C , Yi Q , Quan H , Li W , Wang Y , Lin X , Wang M , Wang J |
| Ref : Bioorg Chem , 162 :108587 , 2025 |
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Abstract :
In this study, a novel fluorescent probe DCI-Gal-Bz toward CEs was developed. DCI-Gal-Bz exhibits excellent sensitivity and selectivity toward CEs, which is not disturbed by physiologically relevant interferences. DCI-Gal-Bz could specifically distinguish hepatocytes from A549, HeLa, and SGC-7901 cells, showing good hepatocyte-targeting potential, which is because the terminal galactose of DCI-Gal-Bz can be selectively recognized by HepG2 cells overexpressing ASGPR. DCI-Gal-Bz could effectively monitor CEs activity in APAP-induced liver injury (DILI), and reveal the upregulation of CEs during N-acetylcysteine (NAC) hepatoprotective therapy. In other words, DCI-Gal-Bz can clearly distinguish between healthy, injured and repaired states, being a powerful tool for exploring liver CEs-related diseases. |
| PubMedSearch : Liu_2025_Bioorg.Chem_162_108587 |
| PubMedID: 40381462 |
| Substrate | DCI-Gal-Bz |
Liu M, Ding C, Yi Q, Quan H, Li W, Wang Y, Lin X, Wang M, Wang J (2025)
Carboxylesterase-activated hepatocyte-targeting fluorescent probe for drug-induced liver injury diagnosis
Bioorg Chem
162 :108587
Liu M, Ding C, Yi Q, Quan H, Li W, Wang Y, Lin X, Wang M, Wang J (2025)
Bioorg Chem
162 :108587