Zadlo_2015_Chembiochem_16_677

Reference

Title : Mixed carbonates as useful substrates for a fluorogenic assay for lipases and esterases - Zadlo_2015_Chembiochem_16_677
Author(s) : Zadlo A , Koszelewski D , Borys F , Ostaszewski R
Ref : Chembiochem , 16 :677 , 2015
Abstract :

Mixed carbonates of 7-hydroxy-4-methylcoumarin were shown to be a new class of probe for fluorogenic assays. They are promising substrates for fingerprinting enzyme hydrolytic activity, and proved particularly useful because of the low level of nonspecific degradation and ease of synthesis. They are highly relevant for screening lipase and esterase libraries. These advantages make umbelliferyl carbonates highly suitable substrates for high-throughput screening. Moreover, we report the use of chiral fluorogenic carbonates as enantiopreference probes.

PubMedSearch : Zadlo_2015_Chembiochem_16_677
PubMedID: 25648400

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Citations formats

Zadlo A, Koszelewski D, Borys F, Ostaszewski R (2015)
Mixed carbonates as useful substrates for a fluorogenic assay for lipases and esterases
Chembiochem 16 :677

Zadlo A, Koszelewski D, Borys F, Ostaszewski R (2015)
Chembiochem 16 :677