Title: Effects of local inhibition of locus coeruleus acetylcholinesterase by fasciculin in rats Abo V, Viera L, Silveira R, Dajas F Ref: Neuroscience Letters, 98:253, 1989 : PubMed
The inhibition of locus coeruleus (LC) acetylcholinesterase (AChE) by Fasciculin II (FAS), a novel anticholinesterase peptide from the green mamba (Dendroaspis angusticeps) venom, was studied in rats. FAS was stereotaxically injected (0.5 microliters of a 1 mg/ml solution) in the right LC. The left LC was taken as control. A group of rats received only saline injected with the same procedure. An inhibition of 80% of LC AChE activity was observed 24 h later. Monoamine and metabolite levels were assessed by high-performance liquid chromatography (HPLC) with electrochemical detection. A significant increase of noradrenaline (NA) levels was found in the injected side when compared with controls 24 h after injection. Neither dopamine, serotonin nor their metabolites or the NA metabolite 4-methoxyhydroxyphenylglycol showed any change after FAS injection. Atropine (30 mg/kg, i.p.) did not prevent the NA increase.
Abo V, Viera L, Silveira R, Dajas F (1989) Effects of local inhibition of locus coeruleus acetylcholinesterase by fasciculin in rats Neuroscience Letters98: 253-7
Abo V, Viera L, Silveira R, Dajas F (1989) Neuroscience Letters98: 253-7