Title: Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli Aoyama S, Yoshida N, Inouye S Ref: FEBS Letters, 242:36, 1988 : PubMed
The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.
Aoyama S, Yoshida N, Inouye S (1988) Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli FEBS Letters242: 36-40
Aoyama S, Yoshida N, Inouye S (1988) FEBS Letters242: 36-40