Title: Manipulation of nitrogen levels and mode of cultivation are viable methods to improve the lipid, fatty acids, phytochemical content, and bioactivities in Chlorella minutissima Aremu AO, Nemenyi M, Stirk WA, Ordog V, Van Staden J Ref: J Phycol, 51:659, 2015 : PubMed
The effect of nitrogen (N: low = 2% N or moderate = 10% N) levels and cultivation (photoautotrophic or mixotrophic) modes on the biochemicals in Chlorella minutissima was evaluated using a mass culture system. Moderate N and mixotrophic cultures had higher biomass, protein, carbohydrate content and photosynthetic pigments than the low N and photoautotrophic treatments. In contrast, lipid and fatty acid content of the low N and photoautotrophic treatments were higher than in the moderate N and mixotrophic cultures. More phytochemicals were accumulated in moderate N and mixotrophic cultures which corresponded to better antioxidant capacity in the extracts. The most potent (0.7 mg . mL(-1) ) acetylcholinesterase inhibitory activity was displayed by moderate N and mixotrophic treatment. Approximately 60% of the extracts exhibited a noteworthy antimicrobial activity regardless of the N levels and cultivation modes. Thus, moderate N level enhanced the phytochemicals and biological activities of C. minutissima cultured under a mixotrophic system.
        
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Aremu AO, Nemenyi M, Stirk WA, Ordog V, Van Staden J (2015) Manipulation of nitrogen levels and mode of cultivation are viable methods to improve the lipid, fatty acids, phytochemical content, and bioactivities in Chlorella minutissima J Phycol51: 659-69
Aremu AO, Nemenyi M, Stirk WA, Ordog V, Van Staden J (2015) J Phycol51: 659-69