Paper Report for: Bauer_2006_Proc.Natl.Acad.Sci.U.S.A_103_11417
Reference
Title: Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls Bauer S, Vasu P, Persson S, Mort AJ, Somerville CR Ref: Proc Natl Acad Sci U S A, 103:11417, 2006 : PubMed
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
Bauer S, Vasu P, Persson S, Mort AJ, Somerville CR (2006) Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls Proc Natl Acad Sci U S A103: 11417-22
Bauer S, Vasu P, Persson S, Mort AJ, Somerville CR (2006) Proc Natl Acad Sci U S A103: 11417-22