Title: [Estimation of the irreversible inhibition reaction rate constants and of the concentration of cholinesterase active sites under commensurable concentrations of enzyme and inhibitors] Brestkin AP, Maizel EB, Smirnov OL Ref: Biokhimiia, 40:521, 1975 : PubMed
Kinetic analysis of the interaction of butyrylcholinesterase and the phosphoorganic inhibitor GT-161 [(C2H2O)2P(O)SC2H4+N(CH3)2C6H5-1-] is carried out. Short time incubations of an enzyme and an inhibitor (1-3 sec), even under commensurable concentrations, were shown to enable the rate constants of irreversible enzyme inhibition to be calcualted by the formula for pseudomonomoleuclar reactions. A simple analytical method for the estimation of the enzyme active sites concentrations is proposed.
        
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Brestkin AP, Maizel EB, Smirnov OL (1975) [Estimation of the irreversible inhibition reaction rate constants and of the concentration of cholinesterase active sites under commensurable concentrations of enzyme and inhibitors] Biokhimiia40: 521-5
Brestkin AP, Maizel EB, Smirnov OL (1975) Biokhimiia40: 521-5