Paper Report for: Buyukfirat_2018_Cell.Mol.Biol.(Noisy-le-grand)_64_74
Reference
Title: Comparison of the cytotoxic, genotoxic and apoptotic effects of Sugammadex and Neostigmine on human embryonic renal cell (HEK-293) Buyukfirat E, Koyuncu I, Karahan MA, Binici O, Altay N, Kirmit A, Gonel A Ref: Cellular & Molecular Biology (Noisy-le-grand), 64:74, 2018 : PubMed
Acetylcholinesterase inhibitors, including Neostigmine, have been used to reverse neuromuscular blockage for many years. Sugammadex reverses this blockage using its gamma cyclodextrin ring, a mechanism that differs from that of cholinesterases and so circumvents the side effects of Neostigmine. Although the superiority of Sugammadex to Neostigmine has been outlined in several clinical studies, to our knowledge, there is not any research into cell culture that compares the cytotoxic, genotoxic and apoptotic effects of the two drugs. Hence, this is the first study to compare the cytotoxic, genotoxic and apoptotic effects of different dosages of both drugs on human embryonic renal (HEK-293) cells. In this study, the cytotoxicity, genotoxicity and apoptotic effects of Sugammadex and Neostigmine on HEK-293 cells were analyzed with using the MTT, Comet Assay and Flow Cytometric Annexin-V methods, respectively. The results demonstrate that Neostigmine at 50, 100, 250, and 500 microg/mL is more cytotoxic than equivalent dosages of Sugammadex. Neostigmine at 500 and 1000 microg/mL was found to be more genotoxic, and Neostigmine at 500 microg/mL had a statistically higher risk of causing apoptosis and necrosis than Sugammadex (p<0.05). Neostigmine administered in-vitro in the same doses as Sugammadex had greater cytotoxic, genotoxic and apoptotic effects on HEK-293 cells.
        
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Citations formats
Buyukfirat E, Koyuncu I, Karahan MA, Binici O, Altay N, Kirmit A, Gonel A (2018) Comparison of the cytotoxic, genotoxic and apoptotic effects of Sugammadex and Neostigmine on human embryonic renal cell (HEK-293) Cellular & Molecular Biology (Noisy-le-grand)64: 74-78
Buyukfirat E, Koyuncu I, Karahan MA, Binici O, Altay N, Kirmit A, Gonel A (2018) Cellular & Molecular Biology (Noisy-le-grand)64: 74-78