Title: Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2 Carriere F, Thirstrup K, Hjorth S, Boel E Ref: FEBS Letters, 338:63, 1994 : PubMed
Starting from total pancreatic mRNAs, the classical guinea pig pancreatic lipase was cloned using rapid amplification of 3' and 5' cDNA ends. Internal oligonucleotide primers were designed from a partial cDNA clone including the region coding for the lid domain. Using this strategy, we did not amplify the cDNA corresponding to the pancreatic lipase related protein 2 in which the lid domain is deleted. Amino acid sequences of the classical guinea pig pancreatic lipase and the related protein 2 were compared based on the primary and tertiary structures of the classical human pancreatic lipase. Their distinct physiological roles are discussed in the light of functional amino acid differences.
Carriere F, Thirstrup K, Hjorth S, Boel E (1994) Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2 FEBS Letters338: 63-8