The activation energies of three processes (AT-depended vesiculation, release of potassium ions from erythrocytes and formation of methemoglobin) in erythrocytes incubated in phosphate buffer are measured in the range 4-37 degrees C. It is show that the Arrhenius plots for the first two processes have the same characteristics namely break at temperature around 18 degrees C, and Ea-58 kJ/mol at t > 18 degrees C. These data suggest that the velocity of ATP-depended vesiculation of erythrocytes is determined by the membrane permeability to monovalent cations. The appearance of methemoglobin in extracellular medium is characterized by a distinct value Ea(120 kJ/mol), although kinetics of this process is similar to vesiculation kinetics. Connection between considered processes and interrelation between ATP-depended vesiculation and spontaneous vesiculation are discussed.
        
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Chernitskii EA, Slobozhanina EI, Fedorovich IE, Novitskaia GP (1994) [Vesiculation of erythrocytes during storage and connection of it with other processes in the cell]. [Russian] Biofizika39: 357-61
Chernitskii EA, Slobozhanina EI, Fedorovich IE, Novitskaia GP (1994) Biofizika39: 357-61