Paper Report for: Connelly_2005_Org.Biomol.Chem_3_3260
Reference
Title: Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase Connelly S, Line K, Isupov MN, Littlechild JA Ref: Org Biomol Chem, 3:3260, 2005 : PubMed
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species showed a molecule bound covalently to the active site serine residue. This enzyme complex represented the first structure of a stably bound tetrahedral intermediate for an alpha/beta hydrolase fold enzyme. The structural elucidation of tetrahedral intermediates is important for the understanding of enzymatic mechanism, substrate recognition and enzyme inhibition. In this paper, we report the synthesis and subsequent characterisation of (3aR,7aS)-3a,4,7,7a-tetrahydrobenzo-[1,3]-dioxol-2-one (BD1), the molecule modelled into the Aureobacterium (-) gamma-lactamase active site. This molecule has been confirmed to be an inhibitor and to be displaced from the enzyme by the racemic gamma-lactam substrate.
        
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Connelly S, Line K, Isupov MN, Littlechild JA (2005) Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase Org Biomol Chem3: 3260-2
Connelly S, Line K, Isupov MN, Littlechild JA (2005) Org Biomol Chem3: 3260-2