Paper Report for: Costa_2022_Enzyme.Microb.Technol_163_110167
Reference
Title: Synthesis of organic-inorganic hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL): Improvement of thermal stability and reusability Costa IO, Rios NS, Lima PJM, Goncalves LRB Ref: Enzyme Microb Technol, 163:110167, 2022 : PubMed
Enzyme immobilization is used to improve the application of enzymes, allowing the reuse of biocatalysts and increasing their stability under reaction conditions. Immobilization of enzymes through structures, such as nanoflowers, is an innovative, simple, and low-cost method compared to other techniques. In this context, the main objective of this work is to synthesize hybrid biocatalytic nanostructures, similar to flowers, of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL). The production of nanoflowers occurred by precipitation of lipases with CuCl(2) or CuSO(4) salts for 72sh. However, challenges and obstacles were faced in obtaining effective and practical nanoflowers, such as nanoflowers' low thermal stability and reusability. To overcome these challenges, two conditions were tested: nanoflowers cross-linked with glutaraldehyde and nanoflowers and nanoparticles cross-linked with glutaraldehyde. This last biocatalyst prepared by CuSO(4) precipitation showed better thermal stability (half-life about 230 and 233smin for CALB and TLL, respectively, under incubation at 60s degreesC and pH 7). The CALB biocatalyst retained 70 % of its initial activity (2.31sU) after 10 cycles of hydrolysis. Therefore, this work shows not only the problems and barriers of nanoflowers synthesis, but also the possibility of producing more stable and efficient biocatalysts using improved protocols.
        
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Citations formats
Costa IO, Rios NS, Lima PJM, Goncalves LRB (2022) Synthesis of organic-inorganic hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL): Improvement of thermal stability and reusability Enzyme Microb Technol163: 110167
Costa IO, Rios NS, Lima PJM, Goncalves LRB (2022) Enzyme Microb Technol163: 110167