Title: A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase De La Hoz DM, Doctor BP, Ralston JS, Rush RS, Wolfe AD Ref: Life Sciences, 39:195, 1986 : PubMed
A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.
De La Hoz DM, Doctor BP, Ralston JS, Rush RS, Wolfe AD (1986) A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase Life Sciences39: 195-9
De La Hoz DM, Doctor BP, Ralston JS, Rush RS, Wolfe AD (1986) Life Sciences39: 195-9